HEK-Lucia™ RIG-I Cells
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Cat.code:
hkl-hrigi
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ABOUT
Human RIG-I Lucia luciferase reporter HEK293 cells
HEK-Lucia™ RIG-I cells were generated from HEK-Lucia™ Null cells, HEK293-derived cells that stably express the secreted Lucia luciferase reporter gene.
This reporter gene is under the control of an IFN‑inducible ISG54 promoter enhanced by multimeric IFN‑stimulated response elements (ISRE).
HEK-Lucia™ RIG-I cells stably express high levels of human RIG-I (also known as Ddx58) and respond strongly to cytosolic double-stranded RNAs with an uncapped 5'-di/triphosphate end [1,2] such as 3p-hpRNA and 5’ppp‑dsRNA (see DATA sheet).
HEK-Lucia™ RIG-I and HEK-Lucia™ Null cells can be used to study the role of RIG-I by monitoring IRF-induced Lucia luciferase activity.
The levels of IRF-induced Lucia in cell culture supernatants can be easily monitored using QUANTI-Luc™ 4 Lucia/Gaussia, a Lucia and Gaussia luciferase detection reagent.
Note: HEK-Lucia™ RIG-I and HEK-Lucia™ Null cells endogenously express NOD1, TLR3, and TLR5.
Disclaimer: These cells are for internal research use only and are covered by a Limited Use License (See Terms and Conditions). Additional rights may be available.
SPECIFICATIONS
Specifications
RIG-I
Human
RIG-I activation cellular assays
Complete DMEM (see TDS)
Verified using Plasmotest™
Each lot is functionally tested and validated.
CONTENTS
Contents
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Product:HEK-Lucia™ RIG-I Cells
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Cat code:hkl-hrigi
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Quantity:3-7 x 10^6 cells
- 1 ml of Zeocin® (100 mg/ml)
- 1 ml of blasticidin (10 mg/ml)
- 1 ml of Normocin™ (50 mg/ml)
- 1 tube of QUANTI-Luc™ 4 Reagent, a Lucia luciferase detection reagent (sufficient to prepare 25 ml)
Shipping & Storage
- Shipping method: Dry ice
- Liquid nitrogen vapor
- Upon receipt, store immediately in liquid nitrogen vapor. Do not store cell vials at -80°C.
Storage:
Caution:
DOCUMENTS
Documents
Technical Data Sheet
Validation Data Sheet
Safety Data Sheet
Certificate of analysis
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