Class A (type D) CpG oligonucleotides - Stimulatory human/mouse TLR9 ligands
Class A (also known as type D) CpG ODNs are potent TLR9 agonists characterized by a central, phosphodiester, CpG-containing palindromic motif. Poly(G) sequences on phosphorothioate backbones are attached to the 3′ and 5′ ends. Class A CpG ODNs are known for strongly activating TLR9 from plasmacytoid dendritic cells (pDCs), inducing type I interferon (especially IFN-α) production, while having a limited effect on B cell activation. This class is primarily used in research focused on innate immune activation and antiviral defense mechanisms.
InvivoGen provides three known Class A CpG ODNs:
- ODN 1585: 5’-ggGGTCAACGTTGAgggggg-3’ (20 mer)
- ODN 2216: 5’-ggGGGACGATCGTCgggggg-3’ (20 mer)
- ODN 2336: 5’-gggGACGACGTCGTGgggggg-3’ (21 mer)
Note: Regular and capital letters represent phosphorothioate and phosphodiester bonds, respectively, bold letters represent CpG dinucleotides, underlining indicates palindromic sequence
Key features
- Potent activators of human and mouse TLR9
- Functionally validated using HEK-Blue™ TLR9 cells (/hek-blue-tlr9)
- HPLC-purified, absence of bacterial contamination
- Provided with endotoxin-free water
The prototype CpG-A ODNs are ODN 1585 in mice and ODN 2216 in humans.