Class A CpG ODNs

TLR9 Agonists – Activators of pDCs and inducers of type IFNs

Class A (type D) CpG oligonucleotides - Stimulatory human/mouse TLR9 ligands

Class A (also known as type D) CpG ODNs are potent TLR9 agonists characterized by a central, phosphodiester, CpG-containing palindromic motif. Poly(G) sequences on phosphorothioate backbones are attached to the 3′ and 5′ ends. Class A CpG ODNs are known for strongly activating TLR9 from plasmacytoid dendritic cells (pDCs), inducing type I interferon (especially IFN-α) production, while having a limited effect on B cell activation. This class is primarily used in research focused on innate immune activation and antiviral defense mechanisms.

InvivoGen provides three known Class A CpG ODNs:

  • ODN 1585: 5’-ggGGTCAACGTTGAgggggg-3’ (20 mer)
  • ODN 2216: 5’-ggGGGACGATCGTCgggggg-3’ (20 mer)
  • ODN 2336: 5’-gggGACGACGTCGTGgggggg-3’ (21 mer)
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Note: Regular and capital letters represent phosphorothioate and phosphodiester bonds, respectively, bold letters represent CpG dinucleotides, underlining indicates palindromic sequence 

Key features

  • Potent activators of human and mouse TLR9
  • Functionally validated using HEK-Blue™ TLR9 cells (/hek-blue-tlr9)
  • HPLC-purified, absence of bacterial contamination
  • Provided with endotoxin-free water

The prototype CpG-A ODNs are ODN 1585 in mice and ODN 2216 in humans.