Human Dectin-1 (CLEC7A) Antibody

Mouse IgG1 (clone 22H8) - Recombinant 

ABOUT

Recombinant anti-human Dectin-1 neutralizing and detection antibody

Anti-hDectin-1-mIgG1 is a monoclonal antibody (mAb) targeting human Dectin-1. This antibody was screened for neutralization activity and flow cytometry (see figures). Anti-hDectin-1-mIgG1 was previously extracted from hybridoma cells (clone 22H8). It is now expressed and produced in Chinese hamster ovary (CHO) cells, ensuring reliability and lot-to-lot reproducibility. Anti-hDectin-1-mIgG1 recognizes hDectin-1 isoforms A and B. 

This antibody can be used together with HEK-Blue™ hDectin-1b Cells for screening and neutralization assays to block hDectin-1 signaling in response to stimulation by Dectin ligands.

 

Key features

  • Each lot is functionally tested and validated.
  • The complete sequence of the antibody construct has been verified.
  • The absence of endotoxins is determined by the EndotoxDetect™ assay.

 

Dendritic cell-associated C-type lectin-1 (Dectin-1) is a C-type lectin receptor (CLR) that recognizes β-glucans, glucose polymers found in the cell walls of fungi, and thus plays an important role in antifungal innate immunity. Dectin-1  is encoded by the C-type lectin domain family 7 member A (CLEC7A) gene and alternatively spliced into 2 major isoforms: a full-length A isoform and a ‘stalkless’ B isoform, which do not induce the same response to soluble and particulate β-glucans [1, 2].

More details More details

 

The hybridoma-derived Anti-hDectin-1-IgG (mabg-hdect-2) antibody has been replaced by Anti-hDectin-1-mIgG1 (mab9-hdect1), which is produced by recombinant technology and purified from CHO cells.

 

 

Read our review Read our review on Dectin-1 & β-glucans.

Read our review Read our review on Dectin-1 & inflammasomes.

 

References:

1. Brown G.D. et al., 2003. Dectin-1 mediates the biological effects of beta-glucans. J Exp Med. 197: 1119- 24.
2. Heinsbroek S.E. et al., 2006. C-type Lectin Receptor: Old Friend and New Player. Med Chem. 2017;13(6):536-543.

All products are for research use only, and not for human or veterinary use.

Neutralizing and detection antibody against hDectin-1
Neutralizing and detection antibody against hDectin-1

SPECIFICATIONS

Specifications

Target

Dectin-1, CLEC7A

Target species

Human

Applications

Blocking assays, detection assay

Species
Human
Isotype
mIgG1
kappa
Recommended isotype control
Recombinant Mouse IgG1 Isotype Control Antibody
Clone
22H8
Tag
Tag-free
Source
CHO cells
Purification
Protein G
Formulation buffer

Sodium phosphate buffer, saccharose, glycine, stabilizing agents

Preservative
Azide
Appearance (form)
Lyophilized
Reconstitution buffer
Sterile water (not provided)
Endotoxin

Negative (tested using EndotoxDetect™ assay)

Tested applications

Neutralization assay, flow cytometry

Quality control

Each lot is functionally tested and validated.

CONTENTS

Contents

  • Product: 
    Anti-hDectin-1-mIgG1
  • Cat code: 
    mab9-hdect1
  • Quantity: 
    100 µg

Shipping & Storage

  • Shipping method:  Room temperature

    • Storage:

    • -20°C
    Stability: -20°C for up to 1 year

    Caution:

    • Avoid repeated freeze-thaw cycles

Details

Dectin-1 is a member of the C-type lectin receptor (CLR) family and plays an important role in antifungal innate immunity. In humans and mice, it is alternatively spliced into two major isoforms, a full-length A isoform and a ‘stalkless’ B isoform [1, 2]. It is expressed on phagocytic cells, including macrophages and neutrophils.
Dectin-1 is a specific receptor for β-glucans [2]. β-Glucans are glucose polymers found in the cell wall of fungi, including the yeasts Saccharomyces cerevisiae and Candida albicans. Upon binding to its ligand, Dectin-1 triggers phagocytosis and signaling through the kinase Syk and the adaptors CARD9-Bcl10-Malt1 leading to the production of reactive oxygen species (ROS), activation of NF-κB and subsequent production of pro-inflammatory cytokines [3].
Notably, Dectin-1 signaling has been shown to collaborate with Toll‑like receptor 2 (TLR2) signaling to enhance the responses triggered by each receptor [4].

 

1. Fischer M. et al., 2017. Isoform localization of Dectin-1 regulates the signaling quality of anti-fungal immunity. Eur J Immunol. 47(5):848-859.
2. Hou H. et al., 2017. C-type Lectin Receptor: Old Friend and New Player. Med Chem. 13(6):536-543.
3. Drummond RA. & Lionakis MS., 2016. Mechanistic Insights into the Role of C-Type Lectin Receptor/ CARD9 Signaling in Human Antifungal Immunity. Front Cell Infect Microbiol. 6:39.
4. Romero MM. et al., 2016. Reactive oxygen species production by human dendritic cells involves TLR2 and dectin-1 and is essential for efficient immune response against Mycobacteria. Cell Microbiol. 18(6):875-86.

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