Human Dectin-1a Reporter HEK 293 Cells

SEAP Reporter 293 cells expressing the human Dectin-1a gene

HEK-Blue™ hDectin-1a cells have been engineered to express a secreted alkaline phosphatase (SEAP) in response to stimulation by Dectin-1 ligands. 

Dectin-1 is a C-type lectin receptor (CLR) that recognizes β-glucans, glucose polymers found in the cell walls of fungi, and thus plays an important role in antifungal innate immunity. Dectin-1 is alternatively spliced into 2 major isoforms: a full-length A isoform and a ‘stalkless’ B isoform, which do not induce the same response to soluble and particulate β-glucans [1].

HEK-Blue™ hDectin-1a cells are derived from the human embryonic kidney HEK293 cells which do not naturally express Dectin-1. We have engineered HEK-Blue™ cells that stably express high levels of human Dectin-1a isoform and genes involved in the Dectin-1/NF-κB/SEAP signaling pathway. Levels of SEAP are readily measurable in real-time by a simple colorimetric assay with HEK-Blue™ Detection or QUANTI-Blue™ . In contrast to cells expressing the human Dectin‑1b gene, HEK‑Blue™ hDectin‑1a cells respond to soluble β-glucans, such as laminarin and WGP soluble.

To identify Dectin-1-specific responses, we recommend to use HEK-Blue™ Null1-v cells as a control cell line.

Read our review on C-type lectin receptor.

Reference:

1. Heinsbroek S.E. et al., 2006. C-type Lectin Receptor: Old Friend and New Player. Med Chem. 2017;13(6):536-543.