Mouse Dectin-1b Reporter HEK 293 Cells

SEAP Reporter 293 cells expressing the murine Dectin-1b gene

HEK-Blue™ mDectin-1b cells have been engineered to express a secreted alkaline phosphatase (SEAP) in response to stimulation by Dectin-1 ligands.

Dectin-1 is a C-type lectin receptor (CLR) that recognizes β-glucans, glucose polymers found in the cell walls of fungi, and thus plays an important role in antifungal innate immunity. Dectin-1 is alternatively spliced into 2 major isoforms: a full-length A isoform and a ‘stalkless’ B isoform, which do not induce the same response to soluble and particulate β-glucans[1].

HEK-Blue™ mDectin-1b cells are derived from the human embryonic kidney HEK293 cells which do not naturally express Dectin-1. We have engineered HEK-Blue™ cells that stably express high levels of murine Dectin-1b isoform and genes involved in the Dectin-1/NF-κB/SEAP signaling pathway. Levels of SEAP are readily measurable in real-time by a simple colorimetric assay with HEK-Blue™ Detection or QUANTI-Blue™ . Like cells expressing the human Dectin‑1b gene, HEK‑Blue™ mDectin‑1b cells do not respond to soluble β-glucans, such as laminarin and WGP soluble.

To identify Dectin-1-specific responses, we recommend to use HEK-Blue™ Null1-v cells as a control cell line.

Read our review on Dectin-1 & inflammasomes.

Reference:

1. Heinsbroek S.E. et al., 2006. Expression of functionally different Dectin-1 isoforms by murine macrophages. J Immunol. 176: 5513.