HKCA

Heat-killed preparation of Candida albicans

HKCA activates the β-glucan specific dectin-1 receptor, which is expressed on phagocytes. β-Glucans are glucose polymers found in the cell walls of fungi, such as zymosan (a cell wall preparation of Saccharomyces cerevisiae) and Candida albicans.

Dectin-1 binds and internalizes β-glucans and mediates the activation of NF-κB and subsequent secretion of proinflammatory cytokines.

HKCA is a heat-killed preparation of C.albicans. HKCA activates the β-glucan specific dectin-1 receptor, which is expressed on phagocytes [1].

β-Glucans are glucose polymers found in the cell walls of fungi, such as zymosan (a cell wall preparation of Saccharomyces cerevisiae) and Candida albicans.

Dectin-1 binds and internalizes β-glucans and mediates the production of reactive oxygen species (ROS), activation of NF-κB and subsequent secretion of proinflammatory cytokines.

However, it is now clear that its β-glucan moiety triggers NF-κB activation only through Dectin-1 as treatment with hot alkali or organic solvents abrogates the TLR2-dependent response [2,3].

References:

1. Brown GD. et al., 2003. Dectin-1 mediates the biological effects of beta-glucans. J Exp Med. 197: 1119- 24.
2. Gantner BN. et al., 2003. Collaborative induction of inflammatory respons- es by dectin-1 and Toll- likereceptor 2. J Exp Med. 197: 1107- 17.
3. ikeda Y. et al., 2008. Dissociation of Toll- like receptor 2-mediated innate immune response to Zymosan by organic solvent-treatment without loss of Dectin-1 reactivity.Biol Pharm Bull.31: 13-8.

Specifications

Specificity: Dectin-1 agonist

Working concentration: 10⁸ cells/ml

Quality Control

• Biological activity has been validated using HEK-Blue™ hDectin-1b cells.
• The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK‑Blue™ TLR4 cells.

Contents

• 10⁹ freeze-dried cells of heat-killed preparation of Candida albicans (HKCA)
• 1 ml sterile endotoxin-free water

HKCA is shipped at room temperature

HKCA should be stored at 4 ̊C