TDB VacciGrade™

Mincle agonist - Synthetic analog of the cord factor

Trehalose-6,6-dibehenate (TDB) is a non-toxic synthetic analog of the mycobacterial cell wall component trehalose 6,6’ dimycolate (TDM, also known as cord factor). TDB was shown to rely on the C-type lectin Mincle and the signaling molecules Syk and Card9 to trigger innate immunity.

TDB VacciGrade™ is a high-quality pre-clinical grade.
TDB VacciGrade™​ is for research use only, and not for human or veterinary use.

Specifications

Description: Mincle agonist VacciGrade™

Polarization of adaptive immune response: Th1 response

Formula: C₅₆H₁₀₆O₁₃

CAS number: 66758-35-8

Molecular weight: 987.43 g/mol

Working concentration: 1 - 100 µg/mouse

Quality control:

• Sterility guaranteed
• The absence of bacterial contamination (lipoproteins & endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cells
• Endotoxin level < 5 EU/mg (measurement by kinetic chromogenic LAL assay)

Contents

TDB VacciGrade™ is provided as a sterile powder.

• 2 x 1 mg TDB VacciGrade™
• 10 ml sterile endotoxin-free physiological water (NaCl 0.9%)

TDB VacciGrade™ is shipped at room temperature.

Store at -20°C.

Lyophilized product is stable for 1 year at -20°C.

VacciGrade™

VacciGrade™ is a high-quality pre-clinical grade. VacciGrade™ products are filter-sterilized (0.2 µm) and filled under strict aseptic conditions in a clean room*. The absence of bacterial contamination is assessed by a sterility test using a pharmacopeia-derived assay. The level of bacterial contaminants (endotoxins and lipoproteins) in each lot is verified using a LAL assay and/or a TLR2 and TLR4 reporter assay.
*Except for LPS VacciGrade™, which is prepared in a laminar flow hood dedicated to LPS.

Details

Chemical structure of TDB:

Trehalose-6,6-dibehenate (TDB) is a non-toxic synthetic analog of the mycobacterial cell wall component trehalose 6,6’ dimycolate (TDM, also known as cord factor).

TDB was shown to rely on the C-type lectin Mincle and the signaling molecules Syk and Card9 to trigger innate immunity [1]. Incorporation of TDB combined with the synthetic amphiphilic cationic lipid compound dimethyldioctadecylammonium (DDA) into liposomes, known as CAF01, has been shown to strongly enhance cellular and humoral responses against a protein antigen [2]. Adjuvanticity of the cationic DDA:TDB liposomes and sustained protection against disease challenge has been demonstrated in particular with a tuberculosis vaccine candidate [3, 4], and has good potential for application in a range of other diseases [5].

1. Schoenen H, et al., 2010. Cutting edge: Mincle is essential for recognition and adjuvanticity of the mycobacterial cord factor and its synthetic analog trehalose-dibehenate. J Immunol. ;184(6):2756-60.
2. Davidsen J, et al., 2005. Characterization of cationic liposomes based on dimethyldioctadecylammonium and synthetic cord factor from M. tuberculosis (trehalose 6,6'-dibehenate)-a novel adjuvant inducing both strong CMI and antibody responses. Biochim Biophys Acta. 1718(1-2):22-31.
3. Holten-Andersen L. et al., 2004. Combination of the cationic surfactant dimethyl dioctadecyl ammonium bromide and synthetic mycobacterial cord factor as an efficient adjuvant for tuberculosis subunit vaccines. Infect Immun. 72(3):1608-17.
4. Christensen D. et al., 2011. Cationic liposomes as vaccine adjuvants. Expert Rev Vaccines. 10(4):513-21.
5. Agger EM. et al., 2008. Cationic liposomes formulated with synthetic mycobacterial cord factor (CAF01): a versatile adjuvant for vaccines with different immunological requirements. PLoS One. 3(9):e3116.