Alhydrogel® adjuvant 2%

Aluminium hydroxide gel | Th2 Response

ABOUT

Vaccine Adjuvant: Aluminium hydroxide gel

Alhydrogel® adjuvant 2%, referred to as alum, is an aluminum hydroxide wet gel suspension. Alum induces a Th2 response by improving the attraction and uptake of antigen by antigen-presenting cells (APCs). It can also activate innate immunity pathways triggered by pattern recognition receptors (PRRs).

In contrast to Adju-Phos®, Alhydrogel® particles have a net positive electrical charge at pH 5-7 and thus are well suited for adsorption of negatively charged antigens (e.g. antigens with isoelectric points below the pH of formulation).

Alhydrogel® adjuvant is sterilized by heating and aseptically filled.

 

Note: Alhydrogel® is a registered trademark that belongs to Croda and is registered in a large number of countries and regions worldwide.

All products are for research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Synonyms
Alum
CAS number
21645-51-2
Product concentration

9.0 – 11.0 mg/ml

Working concentration

1:9 - 1:1 (Alhydrogel®:antigen)

Solubility

Not soluble

Formulation buffer

Ready-to-use, sterile wet gel suspension

Quality control

Aseptically refilled and repacked, Tested for sterility, Tested for pyrogenicity

CONTENTS

Contents

  • Product: 
    Alhydrogel® adjuvant 2%
  • Cat code: 
    vac-alu-50
  • Quantity: 
    50 ml (5 x 10 ml)

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • Room temperature

    Caution:

    • Do not freeze

Details

Alhydrogel adjuvant 2% is an aluminium hydroxide (referred to as alum) wet gel suspension. Alum acts primarily through the formation of a depot at the injection site, enabling enhanced antigen availability, activation of antigen presenting cells (APCs) and uptake by immune cells [1]. It can also activate innate immunity pathways triggered by pattern recognition receptors (PRRs). The NLR pathway has been recently described as an important mechanism in alum adjuvancy [2]. Alum has been shown to activate the NLRP3 inflammasome, although its role in alum-induced antibody response is controversial [3].

 

1. Morefield GL. et al., 2005. Role of aluminum-containing adjuvants in antigen internalization by dendritic cells in vitro. Vaccine 23(13): 1588-95.
2. Li H.  et al., 2008. Cutting edge: Inflammasome activation by alum and alum's adjuvant effect are mediated by NLRP3. J Immunol. 181(1):17-21.
3. Franchi L. & Nuñez G., 2008. The Nlrp3 inflammasome is critical for aluminium hydroxide-mediated IL-1beta secretion but dispensable for adjuvant activity. Eur. J. Immunol. 38(8):2085-9

DOCUMENTS

Documents

Alhydrogel® adjuvant 2%

Safety Data Sheet

Technical Data Sheet

Certificate of analysis

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