ABOUT
Recombinant flagellin from S. typhimurium
Bacterial flagellin, a TLR5 ligand, is a potent T-cell antigen and has potential as a vaccine adjuvant. Unlike other TLR agonists, flagellin tends to produce mixed Th1 and Th2 responses rather than strongly Th1 responses.
Flagellin FliC VacciGrade™ is a high-quality pre-clinical grade.
All products are for internal research use only, and not for human or veterinary use.
VacciGrade™
VacciGrade™ is a high-quality pre-clinical grade, suitable for in vivo studies. VacciGrade™ products are filter-sterilized (0.2 µm) and filled under strict aseptic conditions in a clean room. The absence of bacterial contamination is assessed by a sterility test using a pharmacopeia-derived assay.
SPECIFICATIONS
Specifications
TLR5
1 -10 μg/mouse
500 µg/ml in physiological water
0.2 µm filtration, Sterility guaranteed
Cellular assays
Each lot is functionally tested and validated using cellular assays.
CONTENTS
Contents
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Product:Flagellin FliC VacciGrade™
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Cat code:vac-fla
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Quantity:50 µg
10 ml sterile endotoxin-free physiological water (NaCl 0.9%)
Shipping & Storage
- Shipping method: Room temperature
- -20°C
- Avoid repeated freeze-thaw cycles
Storage:
Caution:
Details
Flagellin FliC is a recombinant flagellin protein encoded by the fliC gene from Salmonella typhimurium. Bacterial flagellin, a TLR5 ligand, is a potent T-cell antigen and has potential as a vaccine adjuvant.
Unlike other TLR agonists, flagellin tends to produce mixed Th1 and Th2 responses rather than strong Th1 responses [1].
It has been demonstrated that flagellin can act as a potent adjuvant in flu vaccines [2, 3].
Furthermore, flagellin can also signal through the NLRC4 inflammasome [4], although it is not known whether this pathway contributes to the adjuvant activity of flagellin.
1. Huleatt J. et al., 2007. Vaccination with recombinant fusion proteins incorporating Toll-like receptor ligands induces rapid cellular and humoral immunity. Vaccine 25(4): 763-75.
2. Mbow ML. et al., 2010. New adjuvants for human vaccines. Curr Opin Immunol. 22(3):411-6.
3. Skountzou I. et al., 2010. Salmonella flagellins are potent adjuvants for intranasally administered whole inactivated influenza vaccine. Vaccine 28(4): 4103-12.
4. Miao EA. & Warren SE., 2010. Innate immune detection of bacterial virulence factors via the NLRC4 inflammasome. J Clin Immunol. 30(4): 502-6
DOCUMENTS
Documents
Technical Data Sheet
Safety Data Sheet
Certificate of analysis
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