Flagellin FliC VacciGrade™ - TLR5-based Adjuvant

Recombinant flagellin from S. typhimurium | Th1/Th2 response

ABOUT

Recombinant flagellin from S. typhimurium

Bacterial flagellin, a TLR5 ligand, is a potent T-cell antigen and has potential as a vaccine adjuvant. Unlike other TLR agonists, flagellin tends to produce mixed Th1 and Th2 responses rather than strongly Th1 responses.

 

Flagellin FliC VacciGrade™ is a high-quality pre-clinical grade.

All products are for internal research use only, and not for human or veterinary use.

VacciGrade™

VacciGrade™ is a high-quality pre-clinical grade, suitable for in vivo studies. VacciGrade™ products are filter-sterilized (0.2 µm) and filled under strict aseptic conditions in a clean room. The absence of  bacterial contamination is assessed by a sterility test using a pharmacopeia-derived assay.

SPECIFICATIONS

Specifications

Specificity

TLR5

Working concentration

1 -10 μg/mouse

Solubility

500 µg/ml in physiological water

Sterility

0.2 µm filtration, Sterility guaranteed

Tested applications

Cellular assays

Quality control

Each lot is functionally tested and validated using cellular assays.

CONTENTS

Contents

  • Product: 
    Flagellin FliC VacciGrade™
  • Cat code: 
    vac-fla
  • Quantity: 
    50 µg
Includes:

10 ml sterile endotoxin-free physiological water (NaCl 0.9%)

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • -20°C

    Caution:

    • Avoid repeated freeze-thaw cycles

Details

Flagellin FliC is a recombinant flagellin protein encoded by the fliC gene from Salmonella typhimurium. Bacterial flagellin, a TLR5 ligand, is a potent T-cell antigen and has potential as a vaccine adjuvant.

Unlike other TLR agonists, flagellin tends to produce mixed Th1 and Th2 responses rather than strong Th1 responses [1].

It has been demonstrated that flagellin can act as a potent adjuvant in flu vaccines [2, 3].

Furthermore, flagellin can also signal through the NLRC4 inflammasome [4], although it is not known whether this pathway contributes to the adjuvant activity of flagellin.

 

1. Huleatt J. et al., 2007. Vaccination with recombinant fusion proteins incorporating Toll-like receptor ligands induces rapid cellular and humoral immunity. Vaccine 25(4): 763-75.
2. Mbow ML. et al., 2010. New adjuvants for human vaccines. Curr Opin Immunol. 22(3):411-6.
3. Skountzou I. et al., 2010. Salmonella flagellins are potent adjuvants for intranasally administered whole inactivated influenza vaccine. Vaccine 28(4): 4103-12.
4. Miao EA. & Warren SE., 2010. Innate immune detection of bacterial virulence factors via the NLRC4 inflammasome. J Clin Immunol. 30(4): 502-6

DOCUMENTS

Documents

Flagellin FliC VacciGrade™

Technical Data Sheet

Safety Data Sheet

Certificate of analysis

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