IFA
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Cat.code:
vac-ifa-10
- Documents
ABOUT
Vaccine Adjuvant: Incomplete Freund′s adjuvant
IFA (Incomplete Freund’s adjuvant) is one of the most commonly used adjuvants in research. It is prepared from non-metabolizable oils.
- IFA can be used to produce water-in-oil emulsions of antigens.
- IFA induces a predominantly Th2-biased response through the formation of a depot at the injection site and the stimulation of antibody-producing plasma cells.
IFA is VacciGrade™, a high-quality pre-clinical grade.
All InvivoGen products are for internal research use only, and not for human or veterinary use.
VacciGrade™
VacciGrade™ is a high-quality pre-clinical grade. VacciGrade™ products are filter-sterilized (0.2 µm) and filled under strict aseptic conditions in a clean room*. The absence of bacterial contamination is assessed by a sterility test using a pharmacopeia-derived assay. The level of bacterial contaminants (endotoxins and lipoproteins) in each lot is verified using a LAL assay and/or a TLR2 and TLR4 reporter assay.
*Except for LPS VacciGrade™, which is prepared in a laminar flow hood dedicated to LPS.
SPECIFICATIONS
Specifications
Th2 response
1:1 (IFA:antigen)
Ready-to-use liquid containing mannide monooleate and paraffin oil
Sterility guaranteed
< 1 EU/ml (measurement by kinetic chromogenic LAL assay)
Adjuvanticity in vivo
Each lot is functionally tested and validated.
CONTENTS
Contents
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Product:IFA
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Cat code:vac-ifa-10
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Quantity:10 mL
Each 10 ml IFA vial contains 1.5 ml Mannide Monooleate and 8.5 ml Paraffin oil.
Shipping & Storage
- Shipping method: Room temperature
- 4°C
- Do not freeze
Storage:
Caution:
Details
IFA (Incomplete Freund’s adjuvant) is one of the most commonly used adjuvants in research.
It is prepared from non-metabolizable oils (paraffin oil and mannide monooleate) [1]. IFA does not contain killed Mycobacterium tuberculosis found in Complete Freund's Adjuvant (CFA) and is thus less inflammatory [1]. IFA induces a predominantly Th2-biased response through the formation of a depot at the injection site and the stimulation of antibody producing plasma cells [2]. It has been suggested that NOD2 modulates the adjuvant effects of IFA [3]. IFA is utilized to produce water-in-oil emulsions of antigens. It is routinely used for boosting immunizations subsequent to CFA. It can also be used for the initial immunization, particularly when a strong antigen is chosen or moderate antibody levels are sufficient.
1. Lindblad EB., 2000. Freund’s Adjuvants. In: Vaccine adjuvants: Preparation Methods and Research Protocols. Humana Press.Totowa, NJ.
2. Petrovsky N. & Aguilar JC., 2004. Vaccine adjuvants: Current state and future trends. Immunol Cell Biol. 82(5): 488-96.
3. Moreira L0. et al., 2008. Modulation of adaptive immunity by different adjuvant-antigen combinations in mice lacking Nod2. Vaccine 26(46): 5808-13.
DOCUMENTS
Documents
Technical Data Sheet
Safety Data Sheet
Certificate of analysis
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