Scleroglucan

Beta-glucan from Sclerotium rolfsii - β(1➝3, 1➝6)-glucan

Scleroglucan is a high molecular weight (>1000 kDa) polysaccharide produced by fermentation of the filamentous fungus Sclerotium rolfsii. It consists of a linear β(1-3) D-glucose backbone with one β(1-6) D-glucose side chain every three main residues.

Scleroglucan is recognized by Dectin-1 [1] and strongly activates HEK-Blue™ Dectin-1 and RAW-Blue™ cells. Detection of β-glucans by Dectin-1 receptor leads to the CARD9-dependent activation of NF-κB and MAP kinases [2].

Similarly to other β-glucans such as schizoplyllan, another high molecular weight β(1->3, 1->6)-glucan, scleroglucan is recognized by Dectin-1 and strongly activates NF-κB (see figure 1 below).

References:

1. Adams EL. et al., 2008. Differential high-affinity interaction of dectin-1 with natural or synthetic glucans is dependent upon primary structure and is influenced by polymer chain length and side-chain branching. J Pharmacol Exp Ther. 325(1):115-23
2. Goodridge HS. et al., 2009. Beta-glucan recognition by the innate immune system. Immunol Rev. 230(1):38-50.

Figures

Figure 1.  Response of HEK-Blue™ hDectin-1b cells to Scleroglucan and Schizophyllan.

HEK-Blue™ hDectin-1b cells were stimulated with 10 µg/ml of Scleroglucan and Schizophyllan. After overnight incubation, the NF-κB response was determined using HEK-Blue™ Detection, a SEAP detection reagent, and by reading the optical density (OD) at 630 nm.

Specifications

Specificity: Dectin-1 ligand

CAS number: 39464-87-4

Partial structure: β(1➝3, 1➝6)-glucan

Contents

• 100 mg scleroglucan provided as a lyophilized powder

Scleroglucan is shipped at room temperature.

Store at room temperature.

Details

Responses to β-glucans.
RAW-Blue™ cells and HEK-Blue™ mDectin-1 cells, which express the mouse dectin-1a gene, were stimulated with 100 µg/ml of various β-glucans.
After 24h incubation, NF-κB activation was assessed by measuring the levels of SEAP (secreted embryonic alkaline phosphatase) using the QUANTI-Blue™ assay.

Stimulatory activity of Dectin-1 ligands:
HEK-Blue™ hDectin-1a and HEK-Blue™ hDectin-1b cells were stimulated with 1 µg/ml or 10 µg/ml Dectin-1 ligands, respectively, and 10 µg/ml TDB.
After 24h incubation, Dectin-1-induced NF-κB activation was assessed by measuring the levels of SEAP using the QUANTI-Blue™ assay.