Recombinant Mouse IgG1 Isotype Control Antibody

 E. coli β-Galactosidase antibody (clone T9C6) - For in vitro and in vivo use

ABOUT

Recombinant mouse IgG1 isotype control mAb against E. coli β-galactosidase

InvivoGen provides a recombinant anti-β-Gal-mIgG1 monoclonal antibody (mAb)  that was previously extracted from hybridoma. It is now expressed and produced in chinese hamster ovary (CHO) cells, ensuring reliability and lot-to-lot reproducibility. Thereby, common hybridoma-related drawbacks such as the generation of non-relevant mAbs containing aberrant light chains are avoided. This control antibody targets E. coli's β-galactosidase (β-Gal), an antigen that is not present in mice. In addition, it displays low ADCC (antibody-dependent cell-mediated cytotoxicity) and no CDC (complement-dependent cytotoxicity) effector functions [1].

The sequence of Anti-β-Gal-mIgG1 is 100% murine (constant and variable regions), as the original clone (clone T9C6) was raised in mice using a proprietary method. This feature allows for reduced immunogenicity and risks of fatal hypersensitivity reactions upon repeated mAb injections into mice [2].

 

InvivoGen provides this antibody in two grades:

  • In vitro use: Anti-β-Gal-mIgG1
  • In vivo use: Anti-β-Gal-mIgG1 InvivoFit™

 

Key features:

  • Targets E. coli β-galactosidase antigen
  • Sequence is 100% murine
  • Murine IgG1 isotype (constant region)
  • Free from non-relevant mAbs found in hybridoma-based productions
  • Produced in animal-free facilities and defined media
  • Low aggregation < 5%
  • InvivoFit™ grade is available

 

References:

1. Bradbury, A. et al., 2018. When monoclonal antibodies are not monospecific: Hybridomas frequently express additional functional variable regions. mAbs, 10(4), 539–546.
2. Yamazaki T. et al., 2005. Blockade of B7-H1 on macrophages suppresses CD4+ T cell proliferation by augmenting IFN-gamma-induced nitric oxide production. J Immunol. 175(3):1586-92.

All products are for research use only, and not for human or veterinary use.

InvivoFit™

InvivoFit™ is a high-quality standard specifically adapted for in vivo studies. 
InvivoFit™ products are filter-sterilized (0.2 µm) and filled under strict aseptic conditions in a clean room. The level of bacterial contaminants (endotoxins and lipoproteins) in each lot is verified using a LAL assay and a TLR2 and TLR4 reporter assay

SPECIFICATIONS

Specifications

Target

E. coli β-galactosidase (β-Gal)

Species
Mouse
Isotype
mIgG1
Clone
T9C6
Tag
Tag-free
Source
CHO cells
Purification
Protein A
Formulation buffer

Sodium phosphate buffer, glycine, saccharose, stabilizing agents

Tested applications

Neutralization assay, flow cytometry, ELISA

Quality control

Each lot is functionally tested and validated.

CONTENTS

Contents

  • Product: 
    Anti-β-Gal-mIgG1
  • Cat code: 
    bgal-mab9-02
  • Quantity: 
    200 µg
Includes:

bgal-mab9-02: 200 µg

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • -20 °C
    Stability: -20°C for up to 1 year

    Caution:

    • Avoid repeated freeze-thaw cycles

DOCUMENTS

Documents

Anti-β-Gal-mIgG1

Technical Data Sheet

Safety Data Sheet

Certificate of analysis

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CUSTOMER SERVICE & TECHNICAL SUPPORT

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