Anti-β-Gal-mIgG1
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Cat.code:
bgal-mab9-02
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ABOUT
Recombinant mouse IgG1 isotype control mAb against E. coli β-galactosidase
InvivoGen provides a recombinant anti-β-Gal-mIgG1 monoclonal antibody (mAb) that was previously extracted from hybridoma. It is now expressed and produced in chinese hamster ovary (CHO) cells, ensuring reliability and lot-to-lot reproducibility. Thereby, common hybridoma-related drawbacks such as the generation of non-relevant mAbs containing aberrant light chains are avoided. This control antibody targets E. coli's β-galactosidase (β-Gal), an antigen that is not present in mice. In addition, it displays low ADCC (antibody-dependent cell-mediated cytotoxicity) and no CDC (complement-dependent cytotoxicity) effector functions [1].
The sequence of Anti-β-Gal-mIgG1 is 100% murine (constant and variable regions), as the original clone (clone T9C6) was raised in mice using a proprietary method. This feature allows for reduced immunogenicity and risks of fatal hypersensitivity reactions upon repeated mAb injections into mice [2].
InvivoGen provides this antibody in two grades:
- In vitro use: Anti-β-Gal-mIgG1
- In vivo use: Anti-β-Gal-mIgG1 InvivoFit™
Key features:
- Targets E. coli β-galactosidase antigen
- Sequence is 100% murine
- Murine IgG1 isotype (constant region)
- Free from non-relevant mAbs found in hybridoma-based productions
- Produced in animal-free facilities and defined media
- Low aggregation < 5%
- InvivoFit™ grade is available
References:
1. Bradbury, A. et al., 2018. When monoclonal antibodies are not monospecific: Hybridomas frequently express additional functional variable regions. mAbs, 10(4), 539–546.
2. Yamazaki T. et al., 2005. Blockade of B7-H1 on macrophages suppresses CD4+ T cell proliferation by augmenting IFN-gamma-induced nitric oxide production. J Immunol. 175(3):1586-92.
All products are for research use only, and not for human or veterinary use.
InvivoFit™
InvivoFit™ is a high-quality standard specifically adapted for in vivo studies.
InvivoFit™ products are filter-sterilized (0.2 µm) and filled under strict aseptic conditions in a clean room. The level of bacterial contaminants (endotoxins and lipoproteins) in each lot is verified using a LAL assay and a TLR2 and TLR4 reporter assay
SPECIFICATIONS
Specifications
E. coli β-galactosidase (β-Gal)
Sodium phosphate buffer, glycine, saccharose, stabilizing agents
Neutralization assay, flow cytometry, ELISA
Each lot is functionally tested and validated.
CONTENTS
Contents
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Product:Anti-β-Gal-mIgG1
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Cat code:bgal-mab9-02
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Quantity:200 µg
bgal-mab9-02: 200 µg
Shipping & Storage
- Shipping method: Room temperature
- -20 °C
- Avoid repeated freeze-thaw cycles
Storage:
Caution:
DOCUMENTS
Documents
Technical Data Sheet
Safety Data Sheet
Certificate of analysis
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