293-Dual™ hSTING-H232 Cells
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Cat.code:
293d-h232
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ABOUT
293-Dual™ hSTING-H232 Cells (ISG-SEAP/KI-[IFN-b]Lucia)
293-Dual™ hSTING-H232 (ISG/KI-IFNb) cells were generated from 293-Dual™ Null (ISG/KI-IFNb) cells by stable transfection of the H232 isoform of human STING (hSTING). R232H has been identified as a natural variant allele of STING occurring in ~14% of the human population [1].
The H232 isoform contains a single amino acid substitution R232H. This isoform has a diminished response to bacterial and metazoan CDNs when compared to the prevalent R232 hSTING allele [1, 2].
H232 has been the most commonly used hSTING variant in published structural studies.
293-Dual™ hSTING-H232 (ISG/KI-IFNb) cells are resistant to blasticidin, hygromycin, and Zeocin®.
References:
1. Yi G. et al., 2013. Single nucleotide polymorphisms of human STING can affect innate immune response to cyclic dinucleotides. PLoS One. 8(10):e77846.
2. Diner E. et al., 2013. The innate immune DNA sensor cGAS produces a noncanonical cyclic dinucleotide that activates human STING. Cell Rep 3(5):1355-61.
Disclaimer: These cells are for internal research use only and are covered by a Limited Use License (See Terms and Conditions). Additional rights may be available.
SPECIFICATIONS
Specifications
Screening of hSTING H232 agonists and inhibitors
Complete DMEM (See TDS)
Verified using Plasmotest™
Each lot is functionally tested and validated.
CONTENTS
Contents
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Product:293-Dual™ hSTING-H232 Cells
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Cat code:293d-h232
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Quantity:3-7 x 10^6 cells
1 ml of Blasticidin (10 mg/ml) 1 ml of Hygromycin (100 mg/ml) 1 ml of Zeocin® (100 mg/ml) 1 ml of Normocin™ (50 mg/ml) 1 ml of QB reagent and 1 ml of QB buffer (sufficient to prepare 100 ml of QUANTI-Blue™ Solution, a SEAP detection reagent) 1 tube of QUANTI-Luc™ 4 Reagent, a Lucia luciferase detection reagent (sufficient to prepare 25 ml)
Shipping & Storage
- Shipping method: Dry ice
- Liquid Nitrogen Vapor
- Upon receipt, store immediately in liquid nitrogen vapor. Do not store cell vials at -80°C.
Storage:
Caution:
DOCUMENTS
Documents
Technical Data Sheet
Validation Data Sheet
Safety Data Sheet
Certificate of analysis
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