Single-stranded RNAs

TLR7 and/or TLR8 activation with ssRNA

InvivoGen offers synthetic single-stranded (ss)RNAs known to activate TLR7 and/or TLR8 - complexed or naked

Single-stranded RNA (ssRNA) has been identified as the natural ligand of the Toll-like receptors TLR7 and TLR8 [1, 2]. ssRNA derived from HIV-1 or the influenza virus were shown to induce the production of proinflammatory cytokines in pDC [3, 4].

This induction was reproduced using polyU or GU-rich oligoribonucleotides (ORNs). To protect them from degradation, these short synthetic ssRNAs can be complexed with cationic lipids.

InvivoGen provides synthetic single-stranded (ss)RNA agonists able to activate human/mouse TLR7 and/or TLR8. The specificity, activity, and potency of each ssRNA/ORN are confirmed using our HEK-Blue™ reporter cell line collection. To ensure batch-to-batch reproducibility, each lot is functionally tested and guaranteed free of bacterial contamination (lipoproteins and endotoxins).

Key Features of InvivoGen's ssRNAs

• Potent activators of TLR7 and/or TLR8
• Activity is tested on human and mouse TLR7 and TLR8
• Ligands are available ready-to-use (complexed) and naked 
• Each lot is highly pure (≥95%) and functionally tested

References:

1. Diebold SS. et al., 2004. Innate antiviral responses by means of TLR7-mediated recognition of single-stranded RNA. Science. 5;303(5663):1529-31
2. Heil F. et al., 2004. Species-specific recognition of single-stranded RNA via toll-like receptor 7 and 8. Science. 5;303(5663):1526-9.
3. Alter G. et al., 2007. Single-Stranded RNA Derived from HIV-1 Serves as a Potent Activator of NK Cells. J Immunol. 178:7658-7666.
4. Hornung V. et al., 2005. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7.Nat Med. 11(3):263-70.