ssPolyU/LyoVec™

TLR7 and/or TLR8 activation with ssRNA

TLR8 Agonist - RNA homopolymer - complexed with LyoVec™

ssPolyU/LyoVec™ is a lyophilized and complexed preparation of single-stranded poly-uridine (ssPolyU), able to activate the Toll-like receptors TLR7 and/or TLR8. 

Single-stranded RNA (ssRNA) has been identified as the natural ligand of TLR7 and TLR8 [1]. ssRNA derived from HIV-1 or the influenza virus were shown to induce the production of proinflammatory cytokines in pDC [2,3]. This induction was reproduced using polyU or GU-rich oligoribonucleotides (ORNs).

Description

InvivoGen's ssPolyU is complexed with the cationic lipid LyoVec™ to protect it from degradation and facilitate its uptake. ssPolyU/LyoVec™ is a specific agonist of human TLR8 as verified by using our HEK-Blue™ reporter cell lines (see figure). Moreover, it activates mouse TLR7 [data not shown].

InvivoGen also offers a non-complexed version ssPolyU Naked.

Key features of ssPolyU/LyoVec™

• Specific activator of human TLR8 
• No activation of human TLR7
• Non-complexed ssPolyU Naked is available
• Complexed with LyoVec™ 
• Each lot of ssPolyU/LyoVec™ is functionally tested

Read our review about TLR7 and TLR8.

References:

1. Heil F. et al., 2004. Species-specific recognition of single-stranded RNA via toll-like receptor 7 and 8. Science. 5;303(5663):1526-9.
2. Alter G. et al., 2007. Single-Stranded RNA Derived from HIV-1 Serves as a Potent Activator of NK Cells. J Immunol. 178:7658-7666.
3. Hornung V. et al., 2005. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7.Nat Med. 11(3):263-70.

Figures

NF-κB response of HEK-Blue™-derived cells to ssPolyU/LyoVec™. HEK-Blue™ cells expressing hTLR7 or hTLR8 were stimulated with increasing concentrations of ssPolyU/LyoVec™. After 24h incubation, the NF-κB-induced SEAP activity was assessed using QUANTI-Blue™. Data are shown as optical density (OD) at 650 nm (mean ± SEM). Of note, HEK-Blue™ Null* comprises data from parental cell lines HEK-Blue Null1 and HEK-Blue Null1-v.

Specifications

Specificity: human TLR8/mouse TLR7 agonist

Working Concentration: 1- 10 μg/ml

Solubility: 0.05 mg/ml in water

Quality control:

• The biological activity of ssPolyU/LyoVec™ has been validated using cellular assays.
• The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ hTLR2 and HEK-Blue™ hTLR4 cells.

Contents

• 4 x 25 μg lyophilized ssPolyU/LyoVec™

Note: Each vial contains 25 μg of ssPolyU complexed with 50 μg LyoVec™.

• 10 ml endotoxin-free water Storage and stability:

ssPolyU/LyoVec™ is provided lyophilized and shipped at room temperature.

Store lyophilized ssPolyU/LyoVec™ at -20 ̊C.

Lyophilized product is stable 1 year when stored -20 ̊C.

Upon resuspension, store ssPolyU/LyoVec™ at 4°C. Resuspended product is stable at 1 week at 4 °C.