ssPolyU Naked
Product | Unit size | Cat. code | Docs. | Qty. | Price | |
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ssPolyU Naked TLR8 Agonist - RNA homopolymer |
Show product |
10 mg |
tlrl-sspu
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TLR7 and/or TLR8 activation with ssRNA
TLR8 Agonist - RNA homopolymer - non-complexed
Poly(U) is a lyophilized preparation of a synthetic single-stranded poly-uridine (ssPolyU), able to activate the Toll-like receptors TLR7 and/or TLR8.
Single-stranded RNA (ssRNA) has been identified as the natural ligand of TLR7 and TLR8 [1]. ssRNA derived from HIV-1 or the influenza virus were shown to induce the production of proinflammatory cytokines in pDC [2,3]. This induction was reproduced using polyU or GU-rich oligoribonucleotides (ORNs).
Description
InvivoGen's ssPolyU Naked is not complexed and therefore, not protected from degradation. Still, ssPolyU Naked is a specific agonist of human TLR8 as verified by using our HEK-Blue™ reporter cell lines (see figure). Moreover, it activates mouse TLR7 [data not shown].
InvivoGen also offers a complexed version ssPolyU/LyoVec™
Key features of ssPolyU Naked
- Specific activator of human TLR8
- No activation of human TLR7
- Complexed ssPolyU/LyoVec™ is available
- Non-complexed
- Each lot of ssPolyU Naked is functionally tested
Read our review about TLR7 and TLR8.
References:
1. Heil F. et al., 2004. Species-specific recognition of single-stranded RNA via toll-like receptor 7 and 8. Science. 5;303(5663):1526-9.
2. Alter G. et al., 2007. Single-Stranded RNA Derived from HIV-1 Serves as a Potent Activator of NK Cells. J Immunol. 178:7658-7666.
3. Hornung V. et al., 2005. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7.Nat Med. 11(3):263-70.
Specifications
Specificity: human TLR8/mouse TLR7 agonist
Working Concentration: 1 - 10 μg/ml
CAS number: 28086-43-3
Solubility: 1 mg/ml in water
Quality control:
- The biological activity of ssPolyU Naked has been validated using cellular assays.
- The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ hTLR2 and HEK-Blue™ hTLR4 cells.
Contents
- 10 mg of lyophilized Poly(U)
- 10 ml of endotoxin-free water
Poly(U) is shipped at room temperature.
Store lyophilized Poly(U) at -20 °C.
Lyophilized product is stable 1 year when stored -20 ̊C.
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