Spike (S) Variants Coding Sequences

SARS-CoV-2 Spike plasmids for cellular expression and protein production

InvivoGen offers a collection of codon-optimized plasmids for the expression of the SARS-CoV-2 Spike (S) as well as various fragments:

• Spike with the ER-retention signal removed*
• RBD (Receptor Binding Domain)
• S1 subunit

These plasmids have been specifically designed for the expression and production of the Spike protein, or fragments, for the screening of novel inhibitors of SARS-CoV-2 host entry (e.g. neutralizing antibodies) by cell-based or non-cell-based assays.

Click here for more information on the SARS-CoV-2 spike.

 

Plasmids for mammalian cell expression

These plasmids encode the full-length Spike protein* with either a functional furin cleavage site, suitable for fusion assays, or an inactive furin cleavage site for optimized surface expression and detection by flow cytometry.

Various SARS-CoV-2 variants are available:

• Original | Wuhan-Hu-1 (Clade 19)
• G614 variant | (Clade 20)
• Alpha | B.1.1.7 variant | United Kingdom (Clade 20I)
• Beta | B.1.351 variant | South Africa (Clade 20H)
• Epsilon | B.1.429 variant | California (Clade 21C)
• Gamma | P.1 variant | Brazil (Clade 20J)
• Iota | B.1.526 variant | New York(Clade 21F)
• Kappa | B.1.617.1 variant | India (Clade 21B)
• Delta | B.1.617.2 variant | India (Clade 21A)
• Lambda | C.37 variant | Peru (Clade GR/452Q.V1)
• Mu | B.1.621 variant | Colombia (Clade 21H)
• Omicron | B.1.1.529/BA.1 variant | South Africa (Clade 21K)
• Omicron | BA.2 variant | South Africa (Clade 21L)
• Omicron | BA.4 & BA.5 variants | South Africa (Clades 22A & 22B)
• Omicron | BQ.1.1 variant | Nigeria (Clade GRA)
• Omicron | XBB.1.5 variant | USA (Clade GRA)
• Omicron | JN.1 variant | USA (Clade GRA)

Click here for more information on SARS-CoV-2 variants

 

Plasmids for expression and production of Spike protein fragments

These plasmids encode Spike fragments from the original isolate first identified in Wuhan. These fragments are tagged with a C-terminal Fc or His tag to facilitate their production. Additionally, they include an exogenous signal sequence to ensure maximum protein secretion and production. The use of these plasmids for tagged protein production has been confirmed in-house.

The following Fc- or His-tagged protein fragments are available:

• Spike RBD (receptor binding domain)
• Spike S1 subunit