Mouse TREX1-KO Reporter RAW 264.7 Cells

TREX1 Knockout IRF-Inducible Lucia luciferase reporter mouse macrophages

RAW-Lucia™ ISG-KO-TREX1 cells were generated from the RAW-Lucia™ ISG cell line, which is derived from the murine RAW 264.7 macrophage cell line, through the stable knockout of the TREX1 gene. TREX1 (also known as DNAse III) is a major cellular 3’5’ exonuclease that plays a crucial role in maintaining immune homeostasis.

RAW-Lucia™ ISG-KO-TREX1 and RAW-Lucia™ ISG cells express a secreted reporter gene, Lucia luciferase, under the control of the I-ISG54 promoter, which is comprised of the IFN-inducible ISG54 promoter enhanced by a multimeric ISRE.

RAW-Lucia™ ISG-KO-TREX1 and RAW-Lucia™ ISG cells can be used to study the role of TREX1 by monitoring IRF-induced Lucia® luciferase activity. The levels of IRF-induced Lucia in the cell culture supernatant can be easily monitored using QUANTI-Luc™ 4 Lucia/Gaussia, a Lucia® and Gaussia luciferase detection reagent.

The response of RAW‑Lucia™ ISG-KO-TREX1 cells to murine type I interferons (IFNs) is unaffected by the knockout of the TREX1 gene. The TREX1 KO appears to have little to no effect on the response to the ligands tested (e.g. VACV70/LyoVec and 2'3'-cGAMP).

RAW-Lucia™ ISG-KO-TREX1 cells are resistant to Zeocin®.