ISG Reporter RAW 264.7 Cells

IRF-Lucia reporter mouse macrophages

RAW-Lucia™ ISG cells were generated from the murine RAW 264.7 macrophage cell line by stable integration of an interferon regulatory factor (IRF)-inducible Lucia luciferase reporter construct.
RAW 264.7 have been reported to express many pattern recognition receptors (PRRs), including the cytosolic DNA sensors (CDS), IFI16 [1], DDX41, AIM2 [2], and cGAS [3], the dsRNA sensor RIG-I [4], and the cyclic dinucleotide sensor STING [5]. Activation of most of these receptors induces the production of type I IFNs through the TBK1-IRF3 axis.

More details about STING

Cell line description:

RAW-Lucia™ ISG cells express the Lucia luciferase gene under the control of an ISG54 minimal promoter in conjunction with five IFN-stimulated response elements. Thus, RAW-Lucia™ ISG cells allow the monitoring of IRF activation by determining the activity of Lucia luciferase. The levels of IRF-induced Lucia luciferase in the cell culture supernatant can be easily monitored using QUANTI-Luc™ 4 Lucia/Gaussia, a Lucia and Gaussia luciferase detection reagent. RAW-Lucia™ ISG cells are resistant to Zeocin®.

RAW-Lucia™ ISG cells are responsive to murine IFN-α and IFN-β but do not respond to their human counterparts. They are also responsive to PRR ligands that trigger the IFN signaling pathway, including CDS ligands, such as transfected double-stranded DNA, RIG-I ligands, such as transfected double-stranded RNA, and STING ligands, such as cGAMP.

Features of RAW-Lucia™ ISG cells:

• Fully functional STING/TBK1/IRF3 signaling pathway
• Readily assessable Lucia luciferase reporter activity
• Functionally tested and guaranteed mycoplasma-free

Applications of RAW-Lucia™ ISG cells:

• Studying the STING/TBK1/IRF3 signaling pathway
• Studying the RIG-I/TBK1/IRF3 signaling pathway

Note: RAW-Blue™ ISG cells, which express the SEAP reporter gene, have been discontinued. Instead, you can use our RAW-Lucia™ ISG cells, which express the Lucia luciferase reporter gene.

References:

1. Kawasaki T. et al., 2011. Recognition of nucleic acids by pattern-recognition receptors and its relevance in autoimmunity. Immunol Rev. 243(1):61-73.
2. Stein SC & Falck-Pedersen E., 2012. Sensing adenovirus infection: activation of interferon regulatory factor 3 in RAW 264.7 cells. J Virol. 86(8):4527-37.
3. Lam E. et al., 2014. Adenovirus Detection by the cGAS/STING/TBK1 DNA Sensing Cascade. J Virol. 88(2):974-81.
4. Melchjorsen J. et al., 2005. Activation of innate defense against a paramyxovirus is mediated by RIG-I and TLR7 and TLR8 in a cell-type-specific manner. J Virol. 79(20):12944-51.
5. Tanaka Y. & Chen ZJ., 2012. STING specifies IRF3 phosphorylation by TBK1 in the cytosolic DNA signaling pathway. Sci Signal. 5(214):ra20.