NF-κB Reporter RAW 264.7 Cells

Mouse macrophage NF-κB-SEAP reporter cell line

RAW-Blue™ cells are designed to monitor the NF-kB and AP-1 responses upon PRR (pattern recognition receptor) stimulation. These cells are derived from the murine RAW 264.7 macrophages. They feature an NF-κB/AP-1-inducible secreted embryonic alkaline phosphatase (SEAP) reporter gene.
RAW 264.7-derived cells endogenously express many pattern-recognition receptors (PRRs), including toll-like receptors (TLRs), NOD-like receptors (NLRs), RIG-I-like receptors (RLRs) and C-type lectin receptors (CLRs) (see figure).

Therefore, RAW-Blue™ cells can be used as:

• TLR reporter cells: they express all TLRs, except TLR5, at different levels
• NOD reporter cells: they express NOD2 and, to a lesser extent, NOD1
• RLR reporter cells: they express RIG-I and MDA-5
• CLR reporter cells: they express Dectin-1

The stimulation with specific agonists of these PRRs induces signaling pathways leading to the activation of NF-κB and AP-1, and the subsequent production of SEAP. Levels of SEAP can be easily monitored using QUANTI-Blue™ solution, a SEAP detection medium (see figure).

Key features:

• Verified expression profile of different PRRs
• Functional validated with a selection of PRR ligands
• Readily monitoring of NF-kB or AP-1 activation by assessing the SEAP activities

Applications:

• Study of various PRRs in innate immunity
• Screening of novel PRR agonists or inhibitors