Invivogen
Menu

RAW-Lucia™ ISG-KO-STING Cells

Product Unit size Cat. code Docs. Qty. Price

RAW-Lucia™ ISG-KO-STING Cells

Murine RAW 264.7 macrophages - STING Knockout IRF-reporter cells

Show product

3-7 x 10e6 cells

rawl-kostg
+-
$1,589
You may also need : Zeocin® | View more associated products

Notification:  This product is for internal research use only. Additional rights may be available. Please visit InvivoGen’s Terms and Conditions.

STING knockout IRF-inducible Lucia luciferase reporter mouse macrophages

RAW-Lucia™ ISG-KO-STING cells were generated from the RAW-Lucia™ ISG cell line, which is derived from the murine RAW 264.7 macrophage cell line, through the stable knockout of the STING gene.
RAW-Lucia™ ISG-KO-STING cells express a secreted reporter gene, Lucia luciferase, under the control of the I-ISG54 promoter which is comprised of the IFN-inducible ISG54 promoter enhanced by a multimeric ISRE.

RAW 264.7 have been reported to express several CDSs, including cGAS [1]. RAW-Lucia™ ISG-KO-STING cells allow the monitoring of IRF activation by determining the activity of Lucia luciferase. The levels of IRF-induced Lucia in the cell culture supernatant can be easily monitored using QUANTI-Luc™ 4 Lucia/Gaussia, a Lucia and Gaussia luciferase detection reagent.

RAW-Lucia™ ISG-KO-STING cells are resistant to Zeocin®.

 

Reference:

1. Lam E. et al., 2014. Adenovirus Detection by the cGAS/STING/TBK1 DNA Sensing Cascade. J Virol. 88(2):974-81.

Figures

Validation of STING knockout by Western blot (Wes™)
Validation of STING knockout by Western blot (Wes™)

Analysis of lysates from the RAW-Lucia™ ISG (WT) and RAW-Lucia™ ISG-KO-STING (KO) cells using Anti-STING, followed by an HRP-conjugated anti-rabbit secondary antibody. The arrow indicates the expected band for the murine STING protein (43 kDa).

IRF induction in RAW-Lucia™ ISG-KO-STING
IRF induction in RAW-Lucia™ ISG-KO-STING

Response of RAW-Lucia™ ISG-KO-STING cells to various stimuli. RAW-Lucia™ ISG-KO-STING and RAW-Lucia™ ISG cells (parental cell line) were incubated with c-di-AMP (3 µg/ml), 2'3'-cGAMP (3 µg/ml), poly(dA:dT)/LyoVec™ (1 µg/ml), and poly(I:C) HMW/LyoVec™ (1 µg/ml). Mouse IFN-α (1x104 U/ml) and IFN-β (1x104 U/ml) serve as positive controls. Non-induced cells (NI) have been included as a negative control. After a 24h incubation, IRFactivation was determined by measuring the relative light units (RLUs) in a luminometer using QUANTI-Luc™, a Lucia luciferase detection reagent. The IRF induction of each ligand is expressed relative to that of mIFN-β at 1x104 U/ml (taken as 100%).

Back to the top

Specifications

Antibiotic resistance: Zeocin®

Growth Medium: DMEM, 4.5 g/l glucose, 10% fetal bovine serum (FBS), 100 µg/ml Normocin™, 2 mM L-glutamine

Quality Control:
STING knockout is verified by functional assays and DNA sequencing to confirm frameshift mutation/deletion.

The cells are guaranteed mycoplasma-free.

This product is covered by a Limited Use License (See Terms and Conditions).

Back to the top

Contents

dry iceShipped on dry ice (Europe, USA, Canada and some areas in Asia)

Back to the top
Customer Service
& Technical Support
Shopping cart is empty