RAW-Lucia™ ISG-KO-RIG-I Cells
Product | Unit size | Cat. code | Docs. | Qty. | Price | |
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RAW-Lucia™ ISG-KO-RIG-I Cells RIG-I Knockout IRF-Lucia Luciferase Reporter Cell Line |
Show product |
3-7 x 10e6 cells |
rawl-korigi
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RIG-I Knockout IRF-Inducible Lucia luciferase reporter mouse macrophages
RAW-Lucia™ ISG-KO-RIG-I cells were generated from the RAW-Lucia™ ISG cell line, which is derived from the murine RAW 264.7 macrophage cell line, through the stable knockout of the RIG-I gene. RIG-I (retinoic-acid-inducible protein 1, also known as Ddx58) is a cytoplasmic RNA helicase, that senses double-stranded RNA (dsRNA), a replication intermediate for RNA viruses, leading to production of type I interferons (IFNs) in infected cells.
RAW-Lucia™ ISG-KO-RIG-I and RAW-Lucia™ ISG cells express a secreted reporter gene, Lucia luciferase, under the control of the I-ISG54 promoter which is comprised of the IFN-inducible ISG54 promoter enhanced by a multimeric ISRE.
RAW-Lucia™ ISG-KO-RIG-I and RAW-Lucia™ ISG cells can be used to study the role of RIG-I by monitoring IRF-induced Lucia luciferase activity. The levels of IRF-induced Lucia in the cell culture supernatant can be easily monitored using QUANTI-Luc™ 4 Lucia/Gaussia, a Lucia and Gaussia luciferase detection reagent.
The response of RAW‑Lucia™ ISG-KO-RIG-I cells to murine type I IFNs is unaffected by the knockout of the RIG-I gene. As expected, the ability of RAW-Lucia™ ISG-KO-RIG-I cells to respond to transfected RNA, such as 5'ppp-dsRNA/LyoVec and poly(I:C) HMW/LyoVec, is greatly reduced.
RAW-Lucia™ ISG-KO-RIG-I cells are resistant to Zeocin®.
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Antibiotic resistance: Zeocin®
Growth Medium: DMEM, 4.5 g/l glucose, 2 mM L-glutamine, 10% (v/v) heat-inactivated fetal bovine serum, 100 U/ml penicillin, 100 μg/ml streptomycin, 100 μg/ml Normocin™ supplemented with Zeocin®
Quality Control:
RIG-I knockout is verified by functional assays and DNA sequencing to confirm frameshift mutation/deletion.
The cells are guaranteed mycoplasma-free.
This product is covered by a Limited Use License (See Terms and Conditions).
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- 1 vial containing 3-7 x 106 cells
- 1 ml of Zeocin® (100 mg/ml)
- 1 ml of Normocin™ (50 mg/ml)
- 1 tube of QUANTI-Luc™ 4 Reagent, a Lucia luciferase detection reagent (sufficient to prepare 25 ml)
Shipped on dry ice (Europe, USA, Canada and some areas in Asia)
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