Mouse RIG-I-KO Reporter RAW 264.7 Cells

RIG-I Knockout IRF-Inducible Lucia luciferase reporter mouse macrophages

RAW-Lucia™ ISG-KO-RIG-I cells were generated from the RAW-Lucia™ ISG cell line, which is derived from the murine RAW 264.7 macrophage cell line, through the stable knockout of the RIG-I gene. RIG-I (retinoic-acid-inducible protein 1, also known as Ddx58) is a cytoplasmic RNA helicase that senses double-stranded RNA (dsRNA), a replication intermediate for RNA viruses, leading to the production of type I interferons (IFNs) in infected cells.

RAW-Lucia™ ISG-KO-RIG-I and RAW-Lucia™ ISG cells express a secreted reporter gene, Lucia luciferase, under the control of the I-ISG54 promoter, which is comprised of the IFN-inducible ISG54 promoter enhanced by a multimeric ISRE.

RAW-Lucia™ ISG-KO-RIG-I and RAW-Lucia™ ISG cells can be used to study the role of RIG-I by monitoring IRF-induced Lucia® luciferase activity. The levels of IRF-induced Lucia in the cell culture supernatant can be easily monitored using QUANTI-Luc™ 4 Lucia/Gaussia, a Lucia and Gaussia luciferase detection reagent.

The response of RAW‑Lucia™ ISG-KO-RIG-I cells to murine type I IFNs is unaffected by the knockout of the RIG-I gene. As expected, the ability of RAW-Lucia™ ISG-KO-RIG-I cells to respond to transfected RNA, such as 5'ppp-dsRNA/LyoVec and poly(I:C) HMW/LyoVec, is greatly reduced.

RAW-Lucia™ ISG-KO-RIG-I cells are resistant to Zeocin®.