Mouse IFI16-KO Reporter RAW 264.7 Cells

IRF-Lucia reporter mouse macrophages

ABOUT

IFI16 Knockout IRF-Inducible Lucia luciferase reporter mouse macrophages

RAW-Lucia™ ISG-KO-IFI16 cells were generated from the RAW-Lucia™ ISG cell line, which is derived from the murine RAW 264.7 macrophage cell line, through the stable knockout of the murine ortholog of IFI16, p204 (also known as IFI204, PYHIN2).
RAW-Lucia™ ISG-KO-IFI16 cells express a secreted reporter gene, Lucia luciferase, under the control of the I-ISG54 promoter which is comprised of the IFN-inducible ISG54 promoter enhanced by a multimeric ISRE.

RAW 264.7 have been reported to express several CDSs, including IFI16 [1]. RAW-Lucia™ ISG-KO-IFI16 cells can be used to study the importance of IFI16 in the induction of type I IFNs in response to nucleic acids. They allow the monitoring of IRF activation by determining the activity of Lucia luciferase. The levels of IRF-induced Lucia in the cell culture supernatant can be easily monitored using QUANTI-Luc™ 4 Lucia/Gaussia, a Lucia and Gaussia luciferase detection reagent.

RAW-Lucia™ ISG-KO-IFI16 cells are resistant to Zeocin®.

 

Reference:

1. Stein SC. & Falck-Pedersen E., 2012. Sensing adenovirus infection: activation of interferon regulatory factor 3 in RAW 264.7 cells. J Virol. 86(8):4527-37.

Disclaimer:  These cells are for internal research use only and are covered by a Limited Use License (See Terms and Conditions). Additional rights may be available.

SPECIFICATIONS

Specifications

Species
Mouse
Tested applications

Screening of PRR agonists or inhibitors

Cell type
Macrophage
Growth properties
Adherent
Tissue origin
Mouse macrophages
Reporter gene
Lucia®
Detection method
Bioluminescence
Antibiotic resistance
Zeocin®
Growth medium

Complete DMEM (see TDS)

Mycoplasma-free

Verified using Plasmotest™

Quality control

Each lot is functionally tested and validated.

CONTENTS

Contents

  • Product: 
    RAW-Lucia™ ISG-KO-IFI16 Cells
  • Cat code: 
    rawl-koif16
  • Quantity: 
    3-7 x 10^6 cells
Includes:
  • 1 ml of Zeocin® (100 mg/ml)
  • 1 ml of Normocin™ (50 mg/ml)
  • 1 tube of QUANTI-Luc™ 4 Reagent, a Lucia luciferase detection reagent (sufficient to prepare 25 ml)

Shipping & Storage

  • Shipping method:  Dry ice
  • Storage:

    • Liquid nitrogen vapor
    Stability: 20 passages

DOCUMENTS

Documents

RAW-Lucia™ ISG-KO-IFI16 Cells

Technical Data Sheet

Safety Data Sheet

Validation Data Sheet

Certificate of analysis

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CUSTOMER SERVICE & TECHNICAL SUPPORT

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