RAW-Lucia™ ISG-KO-IRF3 Cells
Product | Unit size | Cat. code | Docs. | Qty. | Price | |
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RAW-Lucia™ ISG-KO-IRF3 Cells Murine RAW 264.7 macrophages - IRF3 Knockout IRF-Lucia Luciferase Reporter Cells |
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3-7 x 10e6 cells |
rawl-koirf3
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IRF3 knockout reporter mouse macrophages
RAW-Lucia™ ISG-KO-IRF3 cells were generated from the RAW-Lucia™ ISG cell line, which is derived from the murine RAW 264.7 macrophage cell line, through the stable knockout of the IRF3 gene. RAW-Lucia™ ISG-KO-IRF3 cells express a secreted reporter gene, Lucia luciferase, under the control of the I-ISG54 promoter which is comprised of the IFN-inducible ISG54 promoter enhanced by a multimeric ISRE.
IRF-3 is a critical player in the induction of type I IFNs following virus infection. IRF3 is activated by phosphorylation events mediated by the IKK-related kinases TBK-1 and IKKϵ downstream of nucleic acid sensors that detect dsDNA (e.g. cGAS), dsRNA (e.g. RIG-I) or cyclic dinucleotides (STING).
RAW-Lucia™ ISG-KO-IRF3 cells allow the monitoring of IRF activation by determining the activity of Lucia luciferase. The levels of IRF-induced Lucia in the cell culture supernatant can be easily monitored using QUANTI-Luc™ 4 Lucia/Gaussia, a Lucia and Gaussia luciferase detection reagent.
RAW-Lucia ISG-KO-IRF3 cells respond to murine type I IFNs but do not respond to cytosolic nucleic acids, such as poly(dA:dT)/LyoVec ,VACV70/LyoVec, poly(I:C)/LyoVec and 2'3'-cGAMP.
RAW-Lucia™ ISG-KO-IRF3 cells are resistant to Zeocin®.
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Antibiotic resistance: Zeocin®
Growth Medium: DMEM, 4.5 g/l glucose, 2 mM L-glutamine, 10% (v/v) heat-inactivated fetal bovine serum, 100 U/ml penicillin, 100 μg/ml streptomycin, 100 μg/ml Normocin™ supplemented with Zeocin®.
Quality Control:
IRF3 knockout is verified by functional assays and DNA sequencing to confirm frameshift mutation/deletion.
The cells are guaranteed mycoplasma-free.
This product is covered by a Limited Use License (See Terms and Conditions).
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- 1 vial containing 3-7 x 106 cells
- 1 ml of Zeocin® (100 mg/ml)
- 1 ml of Normocin™ (50 mg/ml)
- 1 tube of QUANTI-Luc™ 4 Reagent, a Lucia luciferase detection reagent (sufficient to prepare 25 ml)
Shipped on dry ice (Europe, USA, Canada and some areas in Asia)
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