Poly(I:C) (HMW) / LyoVec™ - RIG-I/MDA5 Agonist

RIG-I/MDA5 Agonist

Poly(I:C) is a synthetic dsRNA polymer. Unlike naked poly(I:C) which is recognized by TLR3, transfected poly(I:C) is sensed by RIG-I/MDA-5 in a cell-type-specific manner [1,2].

Poly(I:C) (HMW)/LyoVec are complexes between poly(I:C) high molecular weight (HMW) and the transfection reagent LyoVec™.

These complexes induce the activation of the RIG-I/MDA-5 signaling pathway at concentrations ranging from 100 ng to 1 µg/ml in C57/WT murine embryonic fibroblasts (MEFs).

As the size of poly(I:C) has been shown to influence RIG-I/MDA-5 activation [3], we also provide the low molecular weight poly(I:C) (LMW)/LyoVec.

References:

1. Gitlin L. et al., 2006. Essential role of mda-5 in type I IFN responses to polyriboinosinic:polyribocytidylic acid and encephelamyocarditis picornavirus. PNAS 103(22):8459-8464.
2. Kato H. et al., 2005. Cell type-specific involvement of RIG-I in antiviral response. Immunity. 23(1):19-28.
3. Kato H et al., 2008. Length-dependent recognition of double-stranded ribonucleic acids by retinoic acid-inducible gene-I and melanoma differentiation-associated gene 5. J. Exp Med. 205(7): 1601-1610.