Poly(dA:dT) - RIG-I & CDS Agonist, AIM2 Inflammasome Inducer

Naked or Complexed with Lyovec™

ABOUT

Synthetic analog of B-DNA

Poly(dA:dT) is a poly(deoxyadenylic-deoxythymidylic) acid sodium salt. It is a repetitive synthetic double-stranded DNA sequence of poly(dA-dT):poly(dT-dA) and a synthetic analog of B-DNA.

Poly(dA:dT)  is recognized by multiple PRRs:
- Sensing by cytosolic DNA sensors (CDS), including cGAS, AIM2, DAI, DDX41, IFI16, and LRRFIP1, triggers the production of type I interferons [1-3].
- Sensing by the cytosolic DNA sensor AIM2 triggers the formation of an inflammasome and the subsequent secretion of IL-1β and IL-18 [4].
- Indirect sensing by the cytosolic RNA sensor RIG-I can occur upon the transcription of Poly(dA:dT) into dsRNA with a 5'triphosphate moiety (5'ppp-dsRNA) by the RNA polymerase III [5,6]. This indirect sensing by RIG-I leads to the production of type I interferons [7].

To achieve stimulation of cytosolic PRRs, Poly(dA:dT) must be delivered into the cytoplasm, for example by using a transfection agent, such as LyoVec™.

 

Key features:

  • Multi-PRR agonist
  • For your convenience, pre-complexed Poly(dA:dT)/ LyoVec™ is also available.
  • Each lot is functionally tested

 

References:

1. Unterholzner L., 2013. The interferon response to intracellular DNA: why so many receptors? Immunobiology. 218(11):1312-21.
2. Zhang Z. et al., 2011. The helicase DDX41 senses intracellular DNA mediated by the adaptor STING in dendritic cells. Nat Immunol. 12(10):959-65.
3. Wu J. et al., 2013. Cyclic GMP-AMP is an endogenous second messenger in innate immune signaling by cytosolic DNA. Science. 339(6121):826-30.
4. Jones JW. et al., 2010. Absent in melanoma 2 is required for innate immune recognition of Francisella tularensis. PNAS, 107(21):9771-6.
5. Ablasser A. et al., 2009. RIG-I-dependent sensing of poly(dA:dT) through the induction of an RNA polymerase III-transcribed RNA intermediate. Nat Immunol. 10(10):1065-72.
6. Chiu YH. et al., 2009. RNA polymerase III detects cytosolic DNA and induces type I interferons through the RIG-I pathway. Cell. 138(3):576-91.
7. Takeshita F. & Ishii KJ., 2008. Intracellular DNA sensors in immunity. Curr Opin Immunol. 20(4):383-8.

All products are for research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

CAS number
86828-69-5
Sequence

poly(deoxyadenylic-deoxythymidylic) acid sodium salt

Working concentration

10 ng - 1 μg/ml for CDS or RLR stimulation
1 µg - 5 μg/ml for Naked Poly(dA:dT) for inflammasome induction

Solubility

2 mg/ml in physiological water (150mM NaCl)

Quality control

Biological activity validated in cellular assays, Absence of bacterial contamination confirmed using HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cells

CONTENTS

Contents

  • Product: 
    Poly(dA:dT) naked
  • Cat code: 
    tlrl-patn
  • Quantity: 
    200 µg
Includes:

1.5 ml sterile endotoxin-free physiological water

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • -20°C

    Caution:

    • Avoid repeated freeze-thaw cycles

DOCUMENTS

Documents

Poly(dA:dT) naked

Technical Data Sheet

Safety Data Sheet

Certificate of analysis

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