Poly(I:C) (LMW) / LyoVec™ - RIG-I/MDA-5 Agonist

dsRNA - complexed with transfection reagent

ABOUT

RIG-I/MDA-5 Agonist

Poly(I:C) is a synthetic dsRNA polymer. Unlike naked poly(I:C) which is recognized by TLR3, transfected poly(I:C) is sensed by RIG-I/MDA-5 in a cell-type-specific manner [1,2].

Poly(I:C) (LMW)/LyoVec are complexes between poly(I:C) low molecular weight (LMW) and the transfection reagent LyoVec™.

These complexes induce the activation of the RIG-I/MDA-5 signaling pathway at concentrations ranging from 100 ng to 1 µg/ml in C57/WT murine embryonic fibroblasts (MEFs).

As the size of poly(I:C) has been shown to influence RIG-I/MDA-5 activation [3], we also provide the high molecular weight Poly(I:C) (HMW)/LyoVec.

 

References:

1. Gitlin L. et al., 2006. Essential role of mda-5 in type I IFN responses to polyriboinosinic:polyribocytidylic acid and encephelamyocarditis picornavirus. PNAS 103(22):8459-8464.
2. Kato H. et al., 2005. Cell type-specific involvement of RIG-I in antiviral response. Immunity. 23(1):19-28.
3. Kato H et al., 2008, Length-dependent recognition of double-stranded ribonucleic acids by retinoic acid-inducible gene-I and melanoma differentiation-associated gene 5. J. Exp Med. 205(7): 1601-1610

All products are for research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Source
Synthetic
Working concentration

100 ng/ml, 1 µg/ml

Solubility

50 µg/µl in water

Endotoxin

<0.05 EU/mg

Quality control

Biological activity validated using cellular assays, Absence of bacterial contamination confirmed using HEK-Blue™ hTLR2 and HEK-Blue™ hTLR4 cells

CONTENTS

Contents

  • Product: 
    Poly(I:C) (LMW) / LyoVec™
  • Cat code: 
    tlrl-picwlv
  • Quantity: 
    100 µg
Includes:

10 ml endotoxin-free water

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • -20°C
    Stability: 1 year at -20°C

DOCUMENTS

Documents

Poly(I:C) (LMW) / LyoVec™

Technical Data Sheet

Safety Data Sheet

Certificate of analysis

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CUSTOMER SERVICE & TECHNICAL SUPPORT

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