c-di-AMP - STING Agonist

Bacterial (canonical) CDN, cyclic [A(3’,5’)pA(3’,5’)p]

ABOUT

STING agonist

Bis-(3'-5')-cyclic dimeric adenosine monophosphate (c-di-AMP) is a bacterial second messenger implicated in the control of cell wall metabolism, osmotic stress responses and sporulation.

Detection of c-di-AMP by the host cytoplasmic surveillance pathway (CSP) is known to elicit type I IFN responses through a signaling axis that involves STING, TBK1 and IRF3 [1, 2].

Involvement of the helicase DDX41 in the recognition of c-di-AMP has been suggested [3].

Recent studies have also demonstrated that c-di-AMP exerts strong adjuvant activities when delivered by the mucosal route [4, 5].

 

References:

1. Jin L. et al., 2011. MPYS is required for IFN response factor 3 activation and type I IFN production in the response of cultured phagocytes to bacterial second messengers cyclic-di-AMP and cyclic-di-GMP. J Immunol. 187(5):2595-601.
2. Burdette DL. et al., 2011. STING is a direct innate immune sensor of cyclic di-GMP. Nature. 478(7370):515-8.
3. Parvatiyar K. et al., 2012 The helicase DDX41 recognizes the bacterial secondary messengers cyclic di-GMP and cyclic di-AMP to activate a type I IFN immune response. Nat. Immunol. 13(12): 1155-61.
4. Ebensen T. et al., 2011. Bis-(3',5')-cyclic dimeric adenosine monophosphate: strong Th1/Th2/Th17 promoting mucosal adjuvant.Vaccine. 29(32):5210-20.
5. Škrnjug I. et al., 2014. The mucosal adjuvant cyclic di-AMP exerts immune stimulatory effects on dendritic cells and macrophages. PLoS One. 9(4):e95728.

All InvivoGen products are for internal research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Source
Synthetic
Synonyms
Cyclic di-adenylate monophosphate
c-di-AMP sodium salt
CAS number
54447-84-6
Chemical formula

C20H22N10O12P2 .2Na

Molecular weight
702.38 g/mol
Working concentration

1-100 µg/ml

Purity
≥ 95% (LC/MS)
Solubility

50 mg/ml in water

Sterility

0.2 µm filtration

Tested applications

Induction of the IFN pathway using cellular assays

Quality control

Each lot is functionally tested and validated.

CONTENTS

Contents

  • Product: 
    c-di-AMP
  • Cat code: 
    tlrl-nacda
  • Quantity: 
    1 mg
Includes:

1 mg lyophilized c-di-AMP, 1.5 ml endotoxin-free water

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • -20°C

    Caution:

    • Avoid repeated freeze-thaw cycles

Details

Structure of c-di-AMP

DOCUMENTS

Documents

c-di-AMP

Technical Data Sheet

Validation Data Sheet

Safety Data Sheet

Certificate of analysis

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CUSTOMER SERVICE & TECHNICAL SUPPORT

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