QUANTI-Star™: ACP5 Detection Reagent

Acid phosphatase 5 (ACP5) detection reagent

ABOUT

Colorimetric-based assay reagent for the detection of acid phosphatase 5 (ACP5)

QUANTI-Star™ is a liquid formulation of a colorimetric enzyme assay. It has been developed to determine the release of acid phosphatase 5 (ACP5) in a biological sample, such as cell culture supernatants.

 

Description

ACP5, also known as tartrate-resistant acid phosphatase (TRAP or TRAPase) is a metalloprotein enzyme, usually located in the lysosome. InvivoGen has developed a secreted (s) version of ACP5 (sACP5) that can be used as a reporter gene.

The level of sACP5 activity in the cell culture supernatant is easily quantified using QUANTI-Star™. This easy-to-use detection reagent contains an ACP5-specific colorimetric substrate, which harbors a phosphate group. Cleavage of this phosphate group by secreted (s)ACP5 leads to the formation of a soluble yellow reaction product, which can be detected at 405 nm using a microplate reader or spectrophotometer. It is therefore combinable with other reporter genes, such as SEAP or Lucia luciferase. ACP5 secretion can be assessed without disturbing cells, thus allowing the repeated sampling of cell cultures for kinetic studies.

For your convenience, InvivoGen offers secreted ACP5 and Lucia-expressing HEK-Lucia-Star™ ARE cells designed to monitor oxidative stress responses.

 

Features and advantages of QUANTI-Star™ ACP5 Detection reagent

  • Convenient, easy, and rapid
  • Cost-effective (1ml = 10 x 96-well plates)
  • Short hands-on-time < 10 min
  • Easy to prepare: just mix QS reagent and QS buffer with water
  • Highly sensitive
  • Allows for high-throughput screening (HTS)

All products are for research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Reporter gene
ACP5
Detection method
Colorimetric
Sample types
HEK-Star™ cell culture supernatant
Sample volume

20 µl

Appearance (form)
Liquid
Applications

Detection and quantification of ACP5 activity

Quality control

Each lot is functionally tested and validated.

CONTENTS

Contents

  • Product: 
    QUANTI-Star™
  • Cat code: 
    rep-qst1
  • Quantity: 
    0.5 ml (100X)
Includes:
  • 0.5 ml of QUANTI-Star™ reagent (100X)
  • 0.5 ml of QUANTI-Star™ buffer (100X)
Notes:

Sufficient to prepare QUANTI‑Star™ Solution for 5 x 96-well plates

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • Upon receipt, store QUANTI-Star™ reagent and QUANTI-Star™ buffer at -20°C.
    Stability: After preparation, the product is stable for 2 weeks at 4°C and up to 2 months at -20°C.

    Caution:

    • Avoid repeated freeze-thaw cycles
    • Protect from light

DOCUMENTS

Documents

QUANTI-Star™

Technical Data Sheet

Safety Data Sheet

Certificate of analysis

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CUSTOMER SERVICE & TECHNICAL SUPPORT

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