Secreted ACP5 Gene

Secreted ACP5 Reporter Gene in expression plasmid

ABOUT

Secreted ACP5 Reporter Gene in expression plasmid

InvivoGen has developed a secreted version of the acid phosphatase 5 (ACP5) that can be used as a reporter gene. ACP5, also known as tartrate-resistant acid phosphatase (TRAP or TRAPase) is a metalloprotein enzyme, usually located in the lysosome.

 

Description

InvivoGens secreted (s)ACP5 features a signaling sequence, that - when expressed in mammalian or insect cells - mediates the export of the sACP5 protein from within the cell to the surrounding culture medium. The level of ACP5 activity in the cell culture supernatant is easily quantified using QUANTI-Star™, our easy-to-use detection reagent. 

Within the pUNO1 plasmid, the sACP5 gene expression is driven by the EF-1α/HTLV composite promoter that combines the elongation factor 1 alpha core promoter and the 5’untranslated region of the Human T-cell Leukemia Virus. The pUNO1-sACP5 plasmid is selectable with Blasticidin in both mammalian cells and bacteria. The gene is flanked by convenient cloning sites for easy subcloning (5’ end: Agel, NcoI. 3’ end: EcoR I site).

The pUNO1-sACP5 plasmid can be subcloned downstream of any promoter of your choice and used in vivo and in vitro to transfect mammalian cells stably or transiently.

All products are for internal research use only, and not for human or veterinary use.

These products are covered by a Limited Use License (See Terms and Conditions).

SPECIFICATIONS

Specifications

Applications

In vitro transfection

ORF size
988 bp
Plasmid backbone
pUNO1
Antibiotic resistance
Blasticidin
Gene promoter
hEF1-HTLV hybrid promoter
Subclone start
Agel / NcoI
Subclone end
EcoRI
Reporter gene
ACP5
Purification
Ion-exchange chromatography
Appearance (form)
Translucent film
Appearance (color)
Colorless
Reconstitution buffer
Sterile water (not provided)
Tested applications

in vitro transfection

Quality control

Plasmid construct has been confirmed by restriction analysis and full‑length open reading frame (ORF) sequencing.

CONTENTS

Contents

  • Product: 
    pUNO1-sACP5
  • Cat code: 
    puno1-sacp5
  • Quantity: 
    20 µg
Includes:
  • 0.5 ml of QUANTI-Star™, an ACP5 detection reagent (sufficient to prepare 50 ml)
  • 1 ml of Blasticidin (10 mg/ml)

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • Upon receipt, store product at -20 °C
    Stability: Reconstituted product is stable for at least 1 year at -20 °C.

    Caution:

    • Avoid repeated freeze-thaw cycles

DOCUMENTS

Documents

pUNO1-sACP5

Technical Data Sheet

Safety Data Sheet

Certificate of analysis

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