pSELECT Lucia-Tag

Expression plasmid with a secreted luciferase (Lucia) Tag

ABOUT

Expression plasmid with a secreted luciferase (Lucia) Tag

pSELECT Lucia-Tag is a family of expression plasmids designed to generate tagged proteins in mammalian cells with the Lucia luciferase in order to detect and quantify the tagged protein of interest by bioluminescence assay.

Lucia is a secreted luciferase with strong bioluminescent activity.The luciferase activity of Lucia luciferase can be detected and quantified directly in the culture medium of transfected cells using InvivoGen's QUANTI-Luc™ detection reagent.

pSELECT Lucia-Tag plasmids are available with Blasticidin or Zeocin™ resistance gene for selection.

All products are for research use only, and not for human or veterinary use.

These products are covered by a Limited Use License (See Terms and Conditions).

SPECIFICATIONS

Specifications

Applications

Bioluminescence assay

Plasmid backbone
pSELECT
Antibiotic resistance
Blasticidin
Gene promoter
Human EF1α/HTLV hybrid promoter
Reporter gene
Lucia®
Purification
Ion-exchange chromatography
Appearance (form)
Translucent film
Appearance (color)
Colorless
Reconstitution buffer
Sterile water (not provided)
Tested applications

In vitro transfection

Quality control

Plasmid construct has been confirmed by restriction analysis and sequencing.

Additional information

N-terminal tag: the tag encompasses the Start Codon and is followed by a multiple cloning site (MCS)

Lucia luciferase-Tag: Detection and quantification of the tagged protein by bioluminescence assay

CONTENTS

Contents

  • Product: 
    pSELECT-NLucia-blasti
  • Cat code: 
    psetb-nlucia
  • Quantity: 
    20 µg
Includes:

2 x 1 ml blasticidin at 10 mg/ml

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • Upon receipt, store product at -20 °C
    Stability: Reconstituted product is stable for at least 1 year at -20 °C.

DOCUMENTS

Documents

pSELECT-NLucia-blasti

Technical Data Sheet

Plasmid Sequence

Safety Data Sheet

Certificate of analysis

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