pFUSE-SEAP-hG2-Fc

Fc-fusion SEAP expression plasmid - Human IgG2

ABOUT

Fc-fusion SEAP expression plasmid - Human IgG2

pFUSE-SEAP-hG2-Fc is a cloning plasmid for generation SEAP-Fc fusion protein. SEAP is a secreted form of human embryonic alkaline phosphatase.

The Fc region of human IgG2 comprises the CH2 and CH3 domains of the IgG2 heavy chain and the hinge region.

The hinge serves as a flexible spacer between the two parts of the Fc-Fusion protein, allowing each part of the molecule to function independently.

Human IgG2 dispays low ADCC and CDC.

 

pFUSE-SEAP-hG2-Fc allows the production of SEAP-human IgG2-Fc fusion proteins.

This plasmid can be used to make recombinant SEAP-Fc fusion proteins or used as a transfection control in experiments with other pFUSE-Fc constructs.

Quantification of SEAP-Fc expression can be determined using QUANTI-Blue™.

All products are for internal research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Applications

Fc-fusion protein generation

Species
Human
Gene
SEAP-hG2-Fc
Plasmid backbone
pFUSE
Antibiotic resistance
Zeocin®
Gene promoter
hEF1-HTLV
Reporter gene
SEAP
Purification
Ion-exchange chromatography
Appearance (form)
Translucent film
Appearance (color)
Colorless
Tested applications

in vitro transfection

Quality control

Plasmid construct has been confirmed by restriction analysis and sequencing.

CONTENTS

Contents

  • Product: 
    pFUSE-SEAP-hG2-Fc
  • Cat code: 
    pfuse-hsp
  • Quantity: 
    20 µg
Includes:

1 ml of Zeocin® (100 mg/ml)

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • -20°C

    Caution:

    • Avoid repeated freeze-thaw cycles

DOCUMENTS

Documents

pFUSE-SEAP-hG2-Fc

Technical Data Sheet

Safety Data Sheet

Plasmid Sequence

Certificate of analysis

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CUSTOMER SERVICE & TECHNICAL SUPPORT

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