pFUSE-CHIg-rhG2
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Cat.code:
pfuse-rhchg2
- Documents
ABOUT
Rhesus monkey IgG2 heavy chain constant region
pFUSE-CHIg-rhG2 is a cloning plasmid that expresses the constant region of the rhesus monkey (Macaca mulatta) IgG2 heavy chain. It contains a multiple cloning site upstream of the constant region to enable cloning of the heavy chain (CH) variable region. This plasmid is designed for antibody generation when co-transfected into mammalian cells with the light chain variable region cloned into pFUSE-CLIg.
pFUSEss-CHIg-rhG2 additionally features the IL2 signal sequence to allow the secretion of recombinant antibodies generated using fragment antigen-binding (Fab) or single-chain variable fragment (scFv) fragments selected from phage display libraries that lack a signal sequence.
Key features of pFUSE-CHIg-rhG2
- Encodes the rhesus monkey IgG2 CH constant region
- Long-lasting expression due to the composite hEF1-HTLV promoter
- Selectable with Zeocin™ in both bacterial and mammalian cells
- Available with or without the IL-2 signal sequence
Specifications
• pFUSE-CHIg-rhG2 features the constant region of the rhesus monkey (Macaca mulatta) IgG2 heavy chain
• pFUSEss-CHIg-rhG2 features the constant region of the rhesus monkey (Macaca mulatta) IgG2 heavy chain and the IL2 signal sequence
All products are for research use only, and not for human or veterinary use.
SPECIFICATIONS
Specifications
In vitro transfection, antibody generation
In vitro transfection
Plasmid construct is confirmed by restriction analysis and full-length open reading frame (ORF) sequencing.
CONTENTS
Contents
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Product:pFUSE-CHIg-rhG2
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Cat code:pfuse-rhchg2
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Quantity:20 µg
1 ml of Zeocin® (100 mg/ml)
Shipping & Storage
- Shipping method: Room temperature
- Upon receipt, store product at -20 °C.
Storage:
DOCUMENTS
Documents
Technical Data Sheet
Safety Data Sheet
Certificate of analysis
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