pFUSE2-CLIg-rhK
| Product | Unit size | Cat. code | Docs. | Qty. | Price | |
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pFUSE2-CLIg-rhK Rhesus monkey kappa |
Show product |
20 µg |
pfuse2-rhclk
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pFUSE2ss-CLIg-rhK Rhesus monkey kappa |
Show product |
20 µg |
pfuse2ss-rhclk
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Light chain expression plasmid - Rhesus monkey kappa
pFUSE2-CLIg-rhK is a cloning plasmid that expresses the constant region of the rhesus monkey (Macaca mulatta) kappa light chain. It contains a multiple cloning site upstream of the constant region to enable cloning of the light chain variable region.
This plasmid is designed for antibody generation when co-transfected into mammalian cells with the heavy chain variable region cloned into pFUSE-CHIg.
pFUSE2ss-CLIg-rhK additionally features the IL2 signal sequence to allow the secretion of recombinant antibodies generated using fragment antigen-binding (Fab) or single-chain variable fragment (scFv) fragments selected from phage display libraries that lack a signal sequence.
Back to the topSpecifications
- pFUSE2-CLIg-rhK features the constant region of the rhesus monkey (Macaca mulatta) Ig kappa light chain
- pFUSE2ss-CLIg-rhK features the constant region of the rhesus monkey (Macaca mulatta) Ig kappa light chain and the IL2 signal sequence
Both plasmids are selectable with blasticidin.
Back to the toppFUSE2 Contents
- 20 µg of lyophilized DNA
- 2 x 1 ml blasticidin at 10 mg/ml
Product is shipped at room temperature.
Lyophilized DNA should be stored at -20 ̊C.
Resuspended DNA should be stored at -20 ̊C and is stable up to 1 year.
Blasticidin is a harmful compound. Refer to safety data sheet for handling instructions.
Store blasticidin at 4°C or -20°C for up to two years. Product is stable 2 weeks at 37°C.
Avoid repeated freeze-thaw cycles.
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