pFUSE-CHIg-ratG2b

Heavy chain expression plasmid - Rat IgG2b

ABOUT

Rat IgG2b heavy chain constant region

 

pFUSE-CHIg-ratG2b is a cloning plasmid that expresses the constant region of the rat IgG2b heavy chain. It contains a multiple cloning site upstream of the constant region to enable cloning of the heavy chain (CH) variable region. This plasmid is designed for antibody generation when co-transfected into mammalian cells with the light chain variable region cloned into pFUSE-CLIg.

pFUSEss-CHIg-ratG2b additionally features the IL2 signal sequence to allow the secretion of recombinant antibodies generated using fragment antigen-binding (Fab) or single-chain variable fragment (scFv)  fragments selected from phage display libraries that lack a signal sequence.

 

Key features of pFUSE-CHIg-ratG2b

  • Encodes the rat IgG2b CH constant region
  • Long-lasting expression due to the composite hEF1-HTLV promoter
  • Selectable with Zeocin™ in both bacterial and mammalian cells
  • Available with or without the IL-2 signal sequence

All products are for internal research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Plasmid backbone
pFUSE
Purification
Ion-exchange chromatography
Reconstitution buffer
Sterile Water
Quality control

Plasmid construct has been confirmed by restriction analysis and sequencing

CONTENTS

Contents

  • Product: 
    pFUSE-CHIg-ratG2b
  • Cat code: 
    pfuse-rtchg2b
  • Quantity: 
    20 µg
Includes:

1 ml of Zeocin® (100 mg/ml)

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • -20°C

    Caution:

    • Avoid repeated freeze-thaw cycles

DOCUMENTS

Documents

pFUSE-CHIg-ratG2b

Technical Data Sheet

Safety Data Sheet

Certificate of analysis

Need a CoA ?

CUSTOMER SERVICE & TECHNICAL SUPPORT

Question about this product ?