pFUSE2-CLIg-ratK

Light chain expression plasmid - Rat kappa

ABOUT

Light chain expression plasmid - Rat kappa

pFUSE2-CLIg-ratK is a cloning plasmid that expresses the constant region of the rat kappa light chain. It contains a multiple cloning site upstream of the constant region to enable cloning of the light chain variable region.

This plasmid is designed for antibody generation when co-transfected into mammalian cells with the heavy chain variable region cloned into pFUSE-CHIg.

pFUSE2ss-CLIg-ratK additionally features the IL2 signal sequence to allow the secretion of recombinant antibodies generated using fragment antigen-binding (Fab) or single-chain variable fragment (scFv)  fragments selected from phage display libraries that lack a signal sequence.

All products are for research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Applications

in vitro or in vivo transfection.

Species
Rat
Plasmid backbone
pFUSE
Antibiotic resistance
Blasticidin
Gene promoter
Human EF1α/HTLV hybrid promoter
Purification
Ion-exchange chromatography
Reconstitution buffer
Endotoxin-free water (not provided)
Tested applications

in vitro transfection

Quality control

Plasmid construct is confirmed by restriction analysis and full-length open reading frame (ORF) sequencing.

CONTENTS

Contents

  • Product: 
    pFUSE2-CLIg-ratK
  • Cat code: 
    pfuse2-rtclk
  • Quantity: 
    20 µg
Includes:

2 x 1 ml blasticidin at 10 mg/ml

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • -20°C
    Stability: Reconstituted product is stable for at least 1 year at -20 °C.

DOCUMENTS

Documents

pFUSE2-CLIg-ratK

Technical Data Sheet

Safety Data Sheet

Certificate of analysis

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CUSTOMER SERVICE & TECHNICAL SUPPORT

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