pFUSE-CHIg-ratG1
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Cat.code:
pfuse-rtchg1
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ABOUT
Rat IgG1 heavy chain constant region
pFUSE-CHIg-ratG1 is a cloning plasmid that expresses the constant region of the rat IgG1 heavy chain. It contains a multiple cloning site upstream of the constant region to enable cloning of the heavy chain (CH) variable region. This plasmid is designed for antibody generation when co-transfected into mammalian cells with the light chain variable region cloned into pFUSE-CLIg.
pFUSEss-CHIg-ratG1 additionally features the IL2 signal sequence to allow the secretion of recombinant antibodies generated using fragment antigen-binding (Fab) or single-chain variable fragment (scFv) fragments selected from phage display libraries that lack a signal sequence.
Key features of pFUSE-CHIg-ratG1
- Encodes the rat IgG1 CH constant region
- Long-lasting expression due to the composite hEF1-HTLV promoter
- Selectable with Zeocin™ in both bacterial and mammalian cells
- Available with or without the IL-2 signal sequence
All products are for research use only, and not for human or veterinary use.
SPECIFICATIONS
Specifications
pFUSE-CHIg-ratG1 is a cloning plasmid that expresses the constant region of the rat IgG1 heavy chain.
Plasmid construct has been confirmed by restriction analysis and sequencing.
Plasmid DNA was purified by ion exchange chromatography.
CONTENTS
Contents
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Product:pFUSE-CHIg-ratG1
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Cat code:pfuse-rtchg1
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Quantity:20 µg
1 ml of Zeocin® (100 mg/ml)
Shipping & Storage
- Shipping method: Room temperature
- Upon receipt, store at -20°C.
- Avoid repeated freeze-thaw cycles
Storage:
Caution:
DOCUMENTS
Documents
Technical Data Sheet
Safety Data Sheet
Certificate of analysis
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