pFUSE-CHIg-mE
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Mouse IgE
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Cat.code:
pfuse-mche
- Documents
ABOUT
Murine IgE heavy chain constant region
pFUSE-CHIg-mE is a cloning plasmid that expresses the constant region of the mouse IgE heavy chain. It contains a multiple cloning site upstream of the constant region to enable cloning of the heavy chain (CH) variable region. This plasmid is designed for antibody generation when co-transfected into mammalian cells with the light chain variable region cloned into pFUSE-CLIg.
pFUSEss-CHIg-mE additionally features the IL2 signal sequence to allow the secretion of recombinant antibodies generated using fragment antigen-binding (Fab) or single-chain variable fragment (scFv) fragments selected from phage display libraries that lack a signal sequence.
Key features of pFUSE-CHIg-mE
- Encodes the murine IgE CH constant region
- Long-lasting expression due to the composite hEF1-HTLV promoter
- Selectable with Zeocin™ in both bacterial and mammalian cells
- Available with or without the IL-2 signal sequence
All products are for research use only, and not for human or veterinary use.
SPECIFICATIONS
Specifications
in vitro or in vivo transfection.
in vitro transfection
Plasmid construct is confirmed by restriction analysis and full-length open reading frame (ORF) sequencing.
CONTENTS
Contents
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Product:pFUSE-CHIg-mE
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Cat code:pfuse-mche
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Quantity:20 µg
1 ml of Zeocin® (100 mg/ml)
Shipping & Storage
- Shipping method: Room temperature
- -20°C
Storage:
DOCUMENTS
Documents
Technical Data Sheet
Safety Data Sheet
Certificate of analysis
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