pFUSE2-CLIg-mL1

Light chain expression plasmid - Murine lambda 1

ABOUT

Light chain expression plasmid - Murine lambda 1

pFUSE2-CLIg-mL1 is a cloning plasmid that expresses the constant region of the mouse lambda 1 light chain. It contains a multiple cloning site upstream of the constant region to enable cloning of the light chain variable region.

This plasmid is designed for antibody generation when co-transfected into mammalian cells with the heavy chain variable region cloned into pFUSE-CHIg.

pFUSE2ss-CLIg-mL1 additionally features the IL2 signal sequence to allow the secretion of recombinant antibodies generated using fragment antigen-binding (Fab) or single-chain variable fragment (scFv)  fragments selected from phage display libraries that lack a signal sequence.

All products are for internal research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Applications

Antibody generation

Species
Mouse
Plasmid backbone
pFUSE2-CLIg
Antibiotic resistance
Blasticidin
Purification
Ion-exchange chromatography
Appearance (form)
Translucent film
Appearance (color)
Colorless
Tested applications

in vitro transfection

Quality control

Plasmid construct has been confirmed by restriction analysis and sequencing.

CONTENTS

Contents

  • Product: 
    pFUSE2-CLIg-mL1
  • Cat code: 
    pfuse2-mcll1
  • Quantity: 
    20 µg
Includes:

2 x 1 ml blasticidin at 10 mg/ml

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • -20°C

    Caution:

    • Avoid repeated freeze-thaw cycles

DOCUMENTS

Documents

pFUSE2-CLIg-mL1

Technical Data Sheet

Plasmid Sequence

Safety Data Sheet

Certificate of analysis

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CUSTOMER SERVICE & TECHNICAL SUPPORT

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