pFUSE-CHIg-hG4e1

pFUSE-CHIg general plasmid map

Human IgG4 heavy chain constant region with reduced Fab-arm exchange

pFUSE-CHIg-hG4e1 is a cloning plasmid that expresses the human IgG4 heavy chain constant region with the S228P mutation for reduced Fab-arm exchange. It contains a multiple cloning site upstream of the constant region to enable cloning of the heavy chain (CH) variable region. This plasmid is designed for antibody generation when co-transfected into mammalian cells with the light chain variable region cloned into pFUSE-CLIg.

pFUSEss-CHIg-hG4e1 additionally features the IL2 signal sequence to allow the secretion of recombinant antibodies generated using fragment antigen-binding (Fab) or single-chain variable fragment (scFv)  fragments selected from phage display libraries that lack a signal sequence.

Key features of pFUSE-CHIg-hG4e1

• Encodes the human IgG4e1 CH constant region (S228P mutation)
• Long-lasting expression due to the composite hEF1-HTLV promoter
• Selectable with Zeocin™ in both bacterial and mammalian cells
• Available with or without the IL-2 signal sequence

Reference:

1. Labrijn AF. et al., 2009. Therapeutic IgG4 antibodies engage in Fab- arm exchange with endogenous human IgG4 in vivo. Nat Biotechnol. 27(8):767-71.

Specifications

• pFUSE-CHIg-hG4e1 features the constant region of the human IgG4 heavy chain with the S228P mutation.

• pFUSEss-CHIg-hG4e1 features the constant region of the human IgG4 heavy chain with the S228P mutation and the IL2 signal sequence

Characteristics of the S228P mutation:
- Reduced Fab-arm exchange

Both plasmids are selectable with Zeocin™.

pFUSE Contents

• 20 µg of lyophilized DNA
• 1 ml of Zeocin® (100 mg/ml)

Product is shipped at room temperature