pFUSE2-CLIg-hL2

Light chain expression plasmid - Human lambda2

ABOUT

Light chain expression plasmid - Human lambda2

pFUSE2-CLIg-hL2 is a cloning plasmid that expresses the constant region of the human lambda 2 light chain. It contains a multiple cloning site upstream of the constant region to enable cloning of the light chain variable region.

This plasmid is designed for antibody generation when co-transfected into mammalian cells with the heavy chain variable region cloned into pFUSE-CHIg.

pFUSE2ss-CLIg-hL2 additionally features the IL2 signal sequence to allow the secretion of recombinant antibodies generated using fragment antigen-binding (Fab) or single-chain variable fragment (scFv)  fragments selected from phage display libraries that lack a signal sequence.

All products are for research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

ORF size
3808 bp
Plasmid backbone
pFUSE2
Antibiotic resistance
Blasticidin
Gene promoter
Human EF1α/HTLV hybrid promoter
Subclone start
AgeI
Subclone end
AvrII
Tested applications

in vitro transfection

Quality control

Plasmid construct is confirmed by restriction analysis and full-length open reading frame (ORF) sequencing.

CONTENTS

Contents

  • Product: 
    pFUSE2-CLIg-hL2
  • Cat code: 
    pfuse2-hcll2
  • Quantity: 
    20 µg
Includes:

2 x 1 ml blasticidin at 10 mg/ml

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • -20°C

DOCUMENTS

Documents

pFUSE2-CLIg-hL2

Technical Data Sheet

Plasmid Sequence

Safety Data Sheet

Certificate of analysis

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CUSTOMER SERVICE & TECHNICAL SUPPORT

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