pFUSE-CHIg-hG1e9

pFUSE-CHIg general plasmid map

Human IgG1 heavy chain constant region with increased phagocytic activity

pFUSE-CHIg-hG1e9 is a cloning plasmid that expresses the human IgG1 heavy chain constant region with S239D / I332E / G236A mutations. It contains a multiple cloning site upstream of the constant region to enable cloning of the heavy chain variable region. This plasmid is designed for antibody generation when co-transfected into mammalian cells with the light chain variable region cloned into pFUSE-CLIg.

pFUSEss-CHIg-hG1e9 additionally features the IL2 signal sequence to allow the secretion of recombinant antibodies generated using fragment antigen-binding (Fab) or single-chain variable fragment (scFv)  fragments selected from phage display libraries that lack a signal sequence.

Key features of pFUSE-CHIg-hG1e9

• Encodes the human IgG1e9 CH constant region (S239D / I332E / G236A mutations)
• Long-lasting expression due to the composite hEF1-HTLV promoter
• Selectable with Zeocin™ in both bacterial and mammalian cells
• Available with or without the IL-2 signal sequence

Reference:

1. Richards J.O. et al., 2008.  Optimization of antibody binding to FcgammaRIIa enhances macrophage phagocytosis of tumor cells. Mol Cancer Ther. 7(8):2517-27.

Specifications

• pFUSE-CHIg-hG1e9 features the constant region of the human IgG1 heavy chain with S239D / I332E / G236A mutations

• pFUSEss-CHIg-hG1e9 features the constant region of the human IgG1 heavy chain with S239D / I332E / G236A mutations and the IL2 signal sequence

Characteristics of S239D / I332E / G236A mutations:
- Increased FcγRIIa/FcγRIIb ratio
- Increased macrophage phagocytosis

Both plasmids are selectable with Zeocin™.

pFUSE Contents

• 20 µg of lyophilized DNA
• 1 ml of Zeocin® (100 mg/ml)

Product is shipped at room temperature