pFUSE-CHIg-hG1e2

pFUSE-CHIg general plasmid map

Human IgG1 heavy chain constant region with an increased serum half-life

pFUSE-CHIg-hG1e2 is a cloning plasmid that expresses the human IgG1 heavy chain constant region with M252Y / S254T / T256E + H433K / N434F mutations. It contains a multiple cloning site upstream of the constant region to enable cloning of the heavy chain (CH) variable region. This plasmid is designed for antibody generation when co-transfected into mammalian cells with the light chain variable region cloned into pFUSE-CLIg.

pFUSEss-CHIg-hG1e2 additionally features the IL2 signal sequence to allow the secretion of recombinant antibodies generated using fragment antigen-binding (Fab) or single-chain variable fragment (scFv)  fragments selected from phage display libraries that lack a signal sequence.

Key features of pFUSE-CHIg-hG1e2

• Encodes the human IgG1e2 CH constant region (M252Y / S254T / T256E + H433K / N434F mutations)
• Long-lasting expression due to the composite hEF1-HTLV promoter
• Selectable with Zeocin™ in both bacterial and mammalian cells
• Available with or without the IL-2 signal sequence

Reference:

1. Vaccaro C. et al., 2005.  Engineering the Fc region of immunoglobulin G to modulate in vivo antibody levels. Nat Biotechnol. 23(10):1283-8.

Specifications

• pFUSE-CHIg-hG1e2 features the constant region of the human IgG1 heavy chain with M252Y / S254T / T256E + H433K / N434F mutations

• pFUSEss-CHIg-hG1e1 features the constant region of the human IgG1 heavy chain with M252Y / S254T / T256E + H433K / N434F mutations and the IL2 signal sequence

Characteristics M252Y / S254T / T256E + H433K / N434F mutations:
- Increased binding to FcRn
- Increased half-life

Both plasmids are selectable with Zeocin™.

pFUSE Contents

• 20 µg of lyophilized DNA
• 1 ml of Zeocin® (100 mg/ml)

Product is shipped at room temperature