Fc-hDectin-1a
| Product | Unit size | Cat. code | Docs. | Qty. | Price | |
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Fc-hDectin-1a Soluble human Dectin-1 receptor |
Show product |
2 x 50 µg |
fc-hdec1a-2
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Soluble human Dectin-1 receptor
Fc-hDectin-1a is a soluble human Dectin-1 receptor constructed by fusing the C-terminal extracellular domain of human Dectin-1a (aa 67-247) to the C-terminus of an engineered human IgG1 Fc domain with a 10 amino acid linker.
Fc-hDectin-1a has an apparent molecular weight of ~55 kDa on SDS-PAGE.
Fc-hDectin-1a is expressed in CHO cells and purified by protein G affinity chromatography.
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Applications: Receptor binding assays, neutralization
Formulation: 0.2 µm filtered solution in phosphate buffered saline (pH 7.4) containing 10% v/v FBS
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- 2 x 50 µg lyophilized Fc-hDectin-1a
Product is shipped at room temperature.
Upon receipt, store at -20°C.
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Details
β-Glucan receptor binding assay:
96-well plates were coated with 0.5 µg of various β-glucans and incubated with 1 μg/ml Fc-hDectin-1a or Fc-mDectin-1a.
After 2 hours, an anti-IgG secondary antibody conjugated to alkaline phosphatase was added.
β-Glucan-Dectin-1 binding was assessed using the QUANTI-Blue™ assay.