Recombinant human IL-1β protein - Bioactive cytokine
| Product | Unit size | Cat. code | Docs. | Qty. | Price | |
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Recombinant human IL-1β (CHO) Cytokine, source: CHO cells - carrier-free |
Show product |
10 µg |
rcyc-hil1b
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Human IL-1β protein - Mammalian cell-expressed, tag-free, carrier-free
Recombinant human IL-1β is a high-quality and biologically active cytokine, validated using proprietary IL-1β reporter cells. This member of the IL-1/TLR cytokine superfamily is produced in CHO cells to ensure protein glycosylation and bona fide 3D structure.
Recombinant human IL-1β can be used together with HEK-Blue™ IL-1β cells for the screening of inhibitory molecules, such as Canakinumab, a therapeutic monoclonal antibody targeting IL-1β (see figures).
IL-1β signaling and biological functions
InvivoGen also offers:
Key features
- Each lot is validated using HEK-Blue™ IL-1β cells
- Endotoxin < 0.1 EU/µg
- 0.2 µm sterile-filtered
Applications
- IL-1β detection and quantification assays (positive control)
- Screening and release assays for antibodies blocking IL-1β signaling
- Screening and release assays for engineered IL-1β proteins
Interleukin 1 beta (IL-1β) is a secreted pro-inflammatory cytokine that generates systemic and local responses to infection and injury. Dysregulated production of IL-1β contributes to inflammatory diseases such as cryopyrin-associated periodic syndrome (CAPS), familial mediterranean fever (FMF), and systemic juvenile idiopathic arthritis (SJIA).
InvivoGen’s products are for research use only, and not for clinical or veterinary use.
Figures
Specifications
Source: Chinese hamster ovary (CHO) cells
Species: Human
Alternative name: Catabolin
Carrier: Carrier-free
Tag: Tag-free
Accession number: P01584
Protein size: 153 a.a. (A117-S269)
Molecular weight: ~ 17 kDa (SDS-PAGE)
Solubility: 100 μg/ml in water
Formulation: Phosphate buffer saline (pH 7.4), 5% saccharose
Sterility: 0.2 µm filtration
Form: Lyophilized
Reconstitution buffer: Endotoxin-free water (provided)
Purity: ≥95% (SDS-PAGE)
Endotoxin level: ≤ 0.1 EU/μg (measurement by kinetic chromogenic LAL assay)
Tested applications: Cellular assays
Quality control: Each lot is functionally tested and validated
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- 10 μg of recombinant human IL-1β provided as a white lyophilized powder
- 1.5 ml endotoxin-free water
Recombinant hIL-1β is shipped at room temperature.
Upon receipt, the product should be stored at -20°C.
Avoid repeated freeze-thaw cycles.
Details
IL‑1 is a secreted pro‑inflammatory cytokine that plays a critical role in immune responses and inflammation [1]. It exists in two distinct isoforms, IL-1α and IL-1β, which are produced as pro-proteins by activated macrophages. IL-1β is cleaved by caspase-1 [2] while IL-1α is cleaved by calcium‑activated calpain or caspase-5 (or its murine ortholog caspase-11) [3]. In contrast to pro‑IL‑1β, pro-IL-1α is active, although cleavage dramatically enhances its bioactivity. IL-1β and IL-1α bind to the same receptor, IL-1R1, triggering the formation of the IL-1R1/IL-1R3/MyD88 complex. This induces signaling leading to the activation of the transcription factors NK-κB and AP-1 with the subsequent inflammatory response [4].
1. Dinarello C., 2018. Overview of the IL-1 family in innate inflammation and acquired immunity. Immunol Rev. 281(1): 8–27.
2. Lopez-Castejon G. & Brough D., 2011. Understanding the mechanism of IL-1β secretion. Cytokine Growth Factor Rev. 22(4):189-95.
3. Wiggins K.A. et al., 2019. IL-1α cleavage by inflammatory caspases of the noncanonical inflammasome controls the senescence-associated secretory phenotype. Aging Cell. 18(3):e12946.
4. Weber A. et al., 2019. Interleukin-1 (IL-1) pathway. Sci Signal. 3(105):cm1.