Recombinant human IL-1β protein (E. coli) - Bioactive cytokine

Human IL-1β protein - E. coli -expressed, tag-free, with HSA

Recombinant human IL-1β is a high-quality and biologically active cytokine, validated using proprietary IL-1β reporter cells. This member of the IL-1/TLR cytokine superfamily is produced in E. coli and thoroughly purified to remove endotoxins.

Recombinant human IL-1β can be used together with HEK-Blue™ IL-1β cells for the screening of inhibitory molecules, such as Canakinumab, a therapeutic monoclonal antibody targeting IL-1β (see figures).

IL-1β signaling and biological functions

Key features

• Each lot is validated using HEK-Blue™ IL-1β cells
• Endotoxin < 0.01 EU/µg
• 0.2 µm sterile-filtered

Applications

• IL-1β detection and quantification assays (positive control)
• Screening and release assays for antibodies blocking IL-1β signaling
• Screening and release assays for engineered IL-1β proteins

Interleukin 1 beta (IL-1β) is a secreted pro-inflammatory cytokine that generates systemic and local responses to infection and injury. Dysregulated production of IL-1β contributes to inflammatory diseases such as cryopyrin-associated periodic syndrome (CAPS), familial mediterranean fever (FMF), and systemic juvenile idiopathic arthritis (SJIA).

 More details

InvivoGen’s products are for research use only, and not for clinical or veterinary use.

Figures

SDS PAGE of the recombinant human (h)IL-1β protein. 3 μg of CHO-derived hIL-1β was loaded on a 12% Mini-PROTEAN® TGX Stain-Free™ Precast Gel (Bio-Rad). Detection was performed as per the manufacturer’s instructions. Its predicted size is ~17 kDa (lower band). The higher band at ~19 kDa corresponds to the glycosylated protein. 

Dose-response in HEK-Blue™ IL-1β cells to recombinant IL-1β cytokines of different sources. Cells were stimulated with increasing concentrations of recombinant human IL-1β, derived from E. coli (blue) or CHO cells (red). After overnight incubation, the NF-κB-induced SEAP activity was determined using QUANTI-Blue™, a SEAP detection reagent. Data are shown as optical density (OD) at 650 nm (mean ± SEM).

Specifications

Source: E. coli

Species: Human

Alternative name: Catabolin

Carrier: HSA (human serum albumin)

Tag: Tag-free

Accession number: P01584

Protein size: 153 a.a. (A117-S269)

Molecular weight: ~ 17 kDa (SDS-PAGE)

Solubility: 100 μg/ml in water

Formulation: Phosphate buffer saline (pH 7.4), 5% saccharose, 2% HSA

Sterility: 0.2 µm filtration

Form:  Lyophilized

Reconstitution buffer:  Endotoxin-free water (provided)

Purity:  ≥95% (SDS-PAGE)

Endotoxin level:  ≤ 0.01 EU/μg (measurement by kinetic chromogenic LAL assay)

Tested applications:  Cellular assays

Quality control: Each lot is functionally tested and validated

Contents

• 10 μg of recombinant human IL-1β provided as a white lyophilized powder
• 1.5 ml endotoxin-free water

 Recombinant hIL-1β is shipped at room temperature.

 Upon receipt, the product should be stored at -20°C.

 Avoid repeated freeze-thaw cycles.

Details

Interleukin 1β (IL-1β) is a soluble pro-inflammatory cytokine that plays a critical role in the host response to infection and injury [1]. It is synthesized as a pro-IL-1β zymogen by activated macrophages and must be cleaved by caspase-1 to generate its mature form [2]. IL-1β binding to the IL-1R1 receptor triggers the formation of the IL-1R1/IL-1R3/MyD88 complex and induces signaling leading to the activation of the transcription factors NK-κB and AP-1 [3].

Due to its role in mediating acute and chronic inflammation, IL-1β has emerged as a therapeutic target for auto-inflammatory diseases [1,4]. 

1. Dinarello C., 2018. Overview of the IL-1 family in innate inflammation and acquired immunity. Immunol Rev. 281(1): 8–27. 
2. Lopez-Castejon G. & Brough D., 2011. Understanding the mechanism of IL-1β secretion. Cytokine Growth Factor Rev. 22(4):189-95.
3. Weber A. et al., 2010. Interleukin-1 (IL-1) pathway. Sci Signal. 3(105):cm1.
4. Dinarello CA., 2011. Interleukin-1 in the pathogenesis and treatment of inflammatory diseases. Blood. 117:3720–3732.