Human IL-4Rα (CD124) Antibody - Dupilumab Biosimilar

Human IgG4 (S228P) - CAS #1190264-60-8

ABOUT

Anti-human IL-4R alpha - Dupilumab biosimilar - CAS #1190264-60-8

Anti-hIL-4R-hIgG4 (S228P) is a biosimilar antibody of Dupilumab, a human interleukin 4 receptor alpha (IL-4Rα) antibody that blocks both IL-4 and IL-13 signaling. This monoclonal antibody (mAb) is FDA-approved for the treatment of asthma, atopic dermatitis, and chronic sinusitis.

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Anti-hIL-4R-hIgG4 (S228P) comprises the variable region of Dupilumab and the IgG4 (S228P) constant region of Dupilumab for low/no effector functions.

This antibody can be used together with HEK-Blue™ IL-4/IL-13 cells for screening and neutralization assays to block IL-4R signaling induced by recombinant human IL-4 or IL-13 (see figure).

Key features

  • Each lot is functionally tested and validated
  • The complete sequence of the antibody construct has been verified
  • Absence of endotoxins determined by the EndotoxDetect assay

All products are for research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Target

IL-4Rα (CD124)

Target species

Human

Applications

Neutralization assay, ELISA, Fc interaction studies

Isotype
hIgG4 (S228P)
kappa
Clone
Dupilumab, REGN-668, SAR-231893
CAS number
1190264-60-8
Source
CHO cells
Production details
Animal-free
Purification
Protein A
Formulation buffer

Sodium phosphate buffer with glycine, saccharose, and stabilizing agents

Preservative
Azide-free
Purity
≥ 95 %
Reconstitution buffer
Sterile water (not provided)
Tested applications

Neutralization assay

Quality control

Each lot is functionally tested and validated.

CONTENTS

Contents

  • Product: 
    Anti-hIL-4R-hIgG4 (S228P)
  • Cat code: 
    hil4r-mab14
  • Quantity: 
    100 µg

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • -20°C
    Stability: Lyophilized product is stable for at least 1 year.

    Caution:

    • Avoid repeated freeze-thaw cycles

Details

Dupilumab background

Dupilumab is a fully human monoclonal antibody (mAb) that targets the human IL-4 receptor alpha subunit (CD124) of the type I and type II IL-4 receptors [1]. It acts like a receptor antagonist and inhibits signaling of both IL-4 and IL-13 [1]. This antibody contains an engineered hinge region mutation (S228P) designed to prevent the exchange of IgG4 molecules [1]. Dupilimab has shown efficacy across multiple Th2 cellular inflammatory disorders and is approved for treating asthma, atopic dermatitis, and chronic sinusitis [1-2]. Due to its favorable safety profile and established clinical use, Dupilumab holds significant promise for amending treatment options for a plethora of dermatologic conditions [3].

 

References:

1. Harb H, Chatila TA, 2020. Mechanisms of Dupilumab. Clin Exp Allergy. 50(1):5-14. 
2. U.S. Food and Drug Administration (FDA) (Report). 2018. New Drug Therapy Approvals 2018.
3. Olbrich H, et al., 2023. Dupilumab in Inflammatory Skin Diseases: A Systematic Review. Biomolecules.13(4):634.

DOCUMENTS

Documents

Anti-hIL-4R-hIgG4 (S228P)

Technical Data Sheet

Validation Data Sheet

Safety Data Sheet

Certificate of analysis

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