Recombinant human IL-4 protein

Recombinant human interleukin-4 protein (with HSA)

Interleukin-4 (IL-4) is a cytokine that plays an important role in regulating inflammation and immune responses. It induces the differentiation of naïve helper T cells to Th2 cells. This cytokine binds the IL-4 receptor that also binds another cytokine interleukin 13 (IL-13), which may explain the overlapping functions of IL-4 and IL-13 [1,2]. Recombinant human IL-4 is intended for use in cell culture applications. It is produced in E. coli.

IL-4 is used to induce its cognate signalling pathway in HEK-Blue™ IL-4/IL-13.

References:

1. Kasaian MT. et al., 2013. An IL-4/IL-13 dual antagonist reduces lung inflammation, airway hyperresponsiveness, and IgE production in mice. Am J Respir Cell Mol Biol.49(1):37-46.
2. David M. et al., 2003. Functional characterization of IL-13 receptor a2 gene promoter: a critical role of the transcription factor STAT6 for regulated expression. Oncogene 22, 3386–3394.

Figures

SDS PAGE of the recombinant human (h)IL-4 cytokine. 1.5 μg of hIL-4 was loaded on a 12% Mini-PROTEAN® TGX Stain-Free™ Precast Gel (Bio-Rad). Detection was performed as per the manufacturer’s instructions. A band was detected at ~15 kDa.

Dose-response in HEK-Blue™ IL-4/IL-13 cells to recombinant IL-4 cytokine. Cells were stimulated with increasing concentrations of recombinant human IL-4. After overnight incubation, the NF-κB-induced SEAP activity was determined using QUANTI-Blue™, a SEAP detection reagent. Data are shown as optical density (OD) at 650 nm (mean ± SEM).

Specifications

Source: E. coli

UniProt ID: P05112

Formulation: recombinant human IL-4 was lyophilized from a 0.2 µM phosphate buffer solution (pH 7.4) containing human serum albumin (HSA).

Purity: >98%, by HPLC and SDS-PAGE.

Molecular mass: ~ 15 kDa

Solubility: 100 μg/ml in water

Endotoxin level: < 0.1 EU/µg as determined by LAL assay

Working Concentration: 0.5 - 100 ng/ml

Bioactivity: Validated using TF-1 cells.

Specific activity: The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.2 ng/ml, corresponding to a specific activity of >5.0 x 10⁶ IU/mg.

Contents

• 10 μg of IL-4 provided as a white lyophilized powder.
• 1.5 ml endotoxin-free water.

IL-4 is shipped at room temperature.

Upon receipt it should be stored at -20°C.