Recombinant human IFN-γ protein

Recombinant human interferon gamma protein (with HSA)

Interferon-gamma (IFN-γ), a Type II interferon, is secreted from CD4⁺ T-helper 1 (Th1) cells and activated natural killer (NK) cells. It plays a role in activating lymphocytes to enhance anti-microbial and anti-tumor effects. In addition, IFN-γ plays a role in regulating the proliferation, differentiation, and response of lymphocyte subsets.

Recombinant human IFN-γ (rhIFN-γ) produced in Escherichia coli is a single, non-glycosylated, polypeptide chain containing 144 amino acids and having a molecular mass of 16879 Daltons.

rhIFN-γ is used to induce its cognate signalling pathway in HEK-Blue™ IFN-γ cells.

Figures

SDS PAGE of the recombinant human (h)IFN-γ cytokine. 1.5 μg of IFN-γ was loaded on a 12% Mini-PROTEAN® TGX StainFree™ Precast Gel (Bio-Rad). Detection was performed as per the manufacturer’s instructions. A band was detected at ~17 kDa.

Dose-response in HEK-Blue™ IFN-γ cells to recombinant hIFN-γ cytokine. Cells were stimulated with increasing concentrations of recombinant human (h)IFN-γ. After overnight incubation, the NF-κB-induced SEAP activity was determined using QUANTI-Blue™, a SEAP detection reagent. Data are shown as optical density (OD) at 650 nm (mean ± SEM).

Specifications

Source: E. coli

Uniprot ID: P01579

Formulation: Lyophilized from a 0.2 μm filtered solution in PBS (pH 7.4) with human serum albumin (HSA) as a carrier protein

Purity: >95%, by SDS-PAGE

Molecular mass: ~ 16.8 kDa

Solubility: 100 μg/ml in water

Endotoxin level: < 0.1 EU/μg protein as determined by the LAL method

Bioactivity: Validated using HEK-Blue IFN-γ cells

Contents

Recombinant human IFN-γ is provided as a white lyophilized powder.

• 20 μg of recombinant human IFN-γ provided as a white lyophilized powder.
• 1.5 ml endotoxin-free water.

Recombinant human IFN-γ is shipped at room temperature.

Upon receipt it should be stored at -20°C.