Recombinant human IL-18 protein - Bioactive cytokine

Human IL-18 protein - E. coli -expressed, tag-free, with HSA

Recombinant human IL-18 is a high-quality and biologically active cytokine, validated using proprietary IL-18 reporter cells. This member of the IL-1/TLR cytokine superfamily is produced in E. coli and thoroughly purified to remove endotoxins.

Recombinant human IL-18 can be used together with HEK-Blue™ IL-18 cells for the screening of inhibitory molecules, such as Camoteskimab, a monoclonal antibody targeting IL-18 (see figures).

IL-18 signaling and biological functions

Key features

• Each lot is validated using HEK-Blue™ IL-18 cells
• Endotoxin < 0.1 EU/µg
• 0.2 µm sterile-filtered

Applications

• Standard for IL-18 detection and quantification assays
• Screening and release assays for antibodies blocking IL-18 signaling
• Screening and release assays for engineered IL-18

Interleukin 18 (IL-18) is a pro-inflammatory cytokine and a powerful inducer of type 1 responses mediated by activated NK, Th1, and CD8+ cytotoxic T cells. Abnormal IL-18 serum concentration is associated with immune-mediated inflammation diseases, including cryopyrin-associated periodic syndrome (CAPS), familial mediterranean fever (FMF), and multiple sclerosis.

 More details

InvivoGen’s products are for research use only, and not for clinical or veterinary use.

Figures

SDS PAGE of the recombinant human (h)IL-18 protein. 1.5 μg of hIL-18 was loaded on a 12% Mini-PROTEAN® TGX Stain-Free™ Precast Gel (Bio-Rad) stained with Coomassie. Detection was performed as per the manufacturer’s instructions. A band was detected at ~18 kDa.

Dose-response in HEK-Blue™ IL-18 cells to recombinant IL-18 cytokine. Cells were stimulated with increasing concentrations of recombinant human IL-18. After overnight incubation, the NF-κBinduced SEAP activity was determined using QUANTI-Blue™, a SEAP detection reagent. Data are shown as optical density (OD) at 650 nm (mean ± SEM).

Specifications

Source: E. coli

Species: Human

Alternative names: Iboctadekin, Interferon gamma-inducing factor

Carrier: HSA (human serum albumin)

Tag: Tag-free

Accession number: Q14116

Protein size: 157 a.a. (T37-D193)

Molecular weight: ~ 18 kDa (SDS-PAGE)

Solubility: 100 μg/ml in water

Formulation: Phosphate buffer saline (pH 7.4), 5% saccharose, 2% HSA

Sterility: 0.2 µm filtration

Form: Lyophilized

Reconstitution buffer: Endotoxin-free water (provided)

Purity: ≥958% (SDS-PAGE)

Endotoxin level: < 0.01 EU/µg (measurement by kinetic chromogenic LAL assay)

Tested applications: Cellular assays

Quality control: Each lot is functionally tested and validated

Contents

• 10 μg of recombinant human IL-18 provided as a white lyophilized powder
• 1.5 ml endotoxin-free water

Recombinant human IL-18 is shipped at room temperature.

Upon receipt, the product should be stored at -20 °C.

 Avoid repeated freeze-thaw cycles.